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Literature summary for 4.2.1.34 extracted from

  • Wang, C.C.; Barker, H.A.
    L-Citramalate hydrolyase (1964), Methods Enzymol., 13, 331-344.
No PubMed abstract available
Search for more literature for 4.2.1.34

Activating Compound

Activating Compound Comment Organism Structure
2,3-dimercapto-1-propanol about half as effective as L-Cys Clostridium tetanomorphum
2-mercaptoethylamine at pH 8.2-8.4 as effective as L-Cys Clostridium tetanomorphum
D-Cys at pH 8.2-8.4 as effective as L-Cys Clostridium tetanomorphum
DL-homocysteine at pH 8.2-8.4 as effective as L-Cys Clostridium tetanomorphum
L-Cys required. The optimal concentration of L-Cys is 0.5-2.0 mM Clostridium tetanomorphum
sulfhydryl compounds required Clostridium tetanomorphum

General Stability

General Stability Organism
freezing and thawing does not significantly decrease the activity of component I, 10-15% loss of activity of component II Clostridium tetanomorphum

Inhibitors

Inhibitors Comment Organism Structure
D-malate
-
 Clostridium tetanomorphum
L-Tartrate
-
 Clostridium tetanomorphum
Mn2+
-
 Clostridium tetanomorphum
succinate
-
 Clostridium tetanomorphum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.59
-
 mesaconate
-
 Clostridium tetanomorphum
0.95
-
 fumarate
-
 Clostridium tetanomorphum
2.4
-
 L-citramalate
-
 Clostridium tetanomorphum
6.7
-
 L-malate
-
 Clostridium tetanomorphum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ maximal activity at 0.2-0.9 mM Clostridium tetanomorphum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 enzyme consists of 2 components, neither component alone possesses hydrolase activity, MW of component I: 46000 Da, gel filtration, MW of component I: 310000 Da, gel filtration, 8 * 37000, SDS-PAGE Clostridium tetanomorphum

Organism

Organism UniProt Comment Textmining
Clostridium tetanomorphum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
component I and II Clostridium tetanomorphum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 of component I and II Clostridium tetanomorphum

Storage Stability

Storage Stability Organism
-10°C, pH 8.4, protein concentration above 1 mg/ml in 0.1 M Tris-HCl buffer, component I is stable for months Clostridium tetanomorphum
-196°C, activity of component II remains constant for at least 8 months Clostridium tetanomorphum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(L)-citramalate
-
 Clostridium tetanomorphum mesaconate + H2O
-
 ?
(S)-malate
-
 Clostridium tetanomorphum fumarate + H2O
-
 ?
Citramalate + H2O
-
 Clostridium tetanomorphum Mesaconate
-
 ?
fumarate + H2O
-
 Clostridium tetanomorphum L-malate
-
 ?
mesaconate
-
 Clostridium tetanomorphum citramalate + H2O
-
 ?

Subunits

Subunits Comment Organism
? enzyme consists of 2 components, neither component alone possesses hydrolase activity, MW of component I: 46000 Da, gel filtration, MW of component I: 310000 Da, gel filtration, 8 * 37000, SDS-PAGE Clostridium tetanomorphum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 2 d, component II is stable in presence of mercaptoethanol, Fe2+, and component I Clostridium tetanomorphum
55
-
 5 min, component II is stable in presence of mercaptoethanol and Fe2+ Clostridium tetanomorphum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 in presence of Cys Clostridium tetanomorphum
8 8.5 in presence of 2-mercaptoethanol Clostridium tetanomorphum

pH Range

pH Minimum pH Maximum Comment Organism
5.7 7.7 about 50% of maximal activity at pH 7.1 and at pH 9.3, in presence of Cys Clostridium tetanomorphum
7.1 9.3 about 50% of maximal activity at pH 7.1 and at pH 9.3, in presence of 2-mercaptoethanol Clostridium tetanomorphum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11 component I is stable Clostridium tetanomorphum
7 8 4°C, 48 h, about 20% loss of activity of component II Clostridium tetanomorphum