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Literature summary for 4.2.1.34 extracted from

  • Suzuki, S.; Osumi, T.; Katsuki, H.
    Properties and metabolic role of mesaconate hydratase of an aerobic bacterium (1977), J. Biochem., 81, 1917-1925.
    View publication on PubMed
Search for more literature for 4.2.1.34

Inhibitors

Inhibitors Comment Organism Structure
PCMB 0.15 mM, complete inhibition. 80% protection by 5 mM S-citramalate Aerobic bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 mesaconate
-
 Aerobic bacterium
0.11
-
 fumarate
-
 Aerobic bacterium
0.45
-
 S-citramalate
-
 Aerobic bacterium
0.56
-
 S-malate
-
 Aerobic bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 gel filtration Aerobic bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aerobic bacterium constitutive enzyme, the enzyme may play a role in the interconversion between S-malate and fumarate in addition to that between S-citramalate and mesaconate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aerobic bacterium
-
-
-

Oxidation Stability

Oxidation Stability Organism
4°C, 90% loss of activity after 24 h in air. Reactivation by Fe2+ and 2-mercaptoethanol Aerobic bacterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1
-
-
 Aerobic bacterium

Storage Stability

Storage Stability Organism
-20°C, in presence of 20% (NH4)2SO4 or 10% glycerol in air, about 80-90% can be reactivated even after several months Aerobic bacterium
4°C, 70% loss of activity after 1 week under N2 Aerobic bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(L)-citramalate
-
 Aerobic bacterium mesaconate + H2O
-
 ?
(S)-malate
-
 Aerobic bacterium fumarate + H2O
-
 ?
Citramalate + H2O
-
 Aerobic bacterium Mesaconate
-
 ?
fumarate + H2O
-
 Aerobic bacterium L-malate
-
 ?
mesaconate
-
 Aerobic bacterium citramalate + H2O
-
 ?
additional information constitutive enzyme, the enzyme may play a role in the interconversion between S-malate and fumarate in addition to that between S-citramalate and mesaconate Aerobic bacterium ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 with mesaconate as substrate Aerobic bacterium
8.7
-
 with fumarate as substrate Aerobic bacterium
9
-
 S-malate as substrate Aerobic bacterium
9.1
-
 with S-citramalate as substrate Aerobic bacterium