General Stability | Organism |
---|---|
no significant loss of activity after repeated freezing and thawing | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R,S)-tartrate | competitive | Pseudomonas putida | |
EDTA | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | reducing conditions: 2 KM-values/biphasic curve, presence of air: only one higher KM-value obtained | Pseudomonas putida | |
0.8 | - |
(R,R)-Tartrate | in the presence of air | Pseudomonas putida | |
2 | - |
(R,R)-Tartrate | elimination of air | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Pseudomonas putida |
Storage Stability | Organism |
---|---|
-20°C, under reduced pressure and minimized contact with air several months | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R,S)-Tartrate | no activity with D-(-)-tartrate, (R,S)-tartrate | Pseudomonas putida | Oxaloacetate + H2O | - |
? | |
L-(+)-Tartrate | no activity with D-(-)-tartrate, (R,S)-tartrate | Pseudomonas putida | Oxaloacetate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | 9.4 | 50% of maximal activity at pH 7.6 and at pH 9.4 | Pseudomonas putida |