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Literature summary for 4.2.1.31 extracted from

  • Ueda, M.; Asano, Y.; Yamada, H.
    Purification and characterization of maleate hydratase from Arthrobacter sp. strain MC12612 (1993), Biosci. Biotechnol. Biochem., 57, 1545-1548.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
additional information activation by Fe2+ and L-cysteine, or other sulfhydryl compounds Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
2,2'-dipyridyl
-
Arthrobacter sp.
CdCl2 24% inhibition at 2 mM Arthrobacter sp.
CuSO4 32% inhibition at 2 mM Arthrobacter sp.
Fe2(SO4)3 41% inhibition at 2 mM Arthrobacter sp.
HgCl2 25% inhibition at 2 mM Arthrobacter sp.
additional information
-
Arthrobacter sp.
o-phenanthroline
-
Arthrobacter sp.
p-chloromercuribenzoate
-
Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.85
-
Maleate
-
Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 353% activation at 2 mM Arthrobacter sp.
Mn2+ 49% activation at 2 mM Arthrobacter sp.
additional information
-
Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. MCI2612
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.55
-
-
Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maleate + H2O
-
Arthrobacter sp. (R)-malate
-
r
maleate + H2O
-
Arthrobacter sp. MCI2612 (R)-malate
-
r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
20% inactivation after 30 min Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5
-
Arthrobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
rapidly inactivated below Arthrobacter sp.