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Literature summary for 4.2.1.31 extracted from

  • Van der Werf, M.J.; Hartmans, S.; Van der Tweel, W.J.J.
    Effect of maleate counterion on malease activity: production of D-malate in a crystal-liquid two-phase system (1995), Enzyme Microb. Technol., 17, 430-436.
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Search for more literature for 4.2.1.31

Application

Application Comment Organism
synthesis biosynthesis of synthons for fine chemicals industry, description of a crystal-liquid two-phase system and soluble metal-organic acid complex system for fermentation which minimizes substrate inhibition and thereby increases efficiency Pseudomonas oleovorans

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 36% inhibition at 100 mM, formation of substrate/product precipitate Pseudomonas oleovorans
Ca2+ 20% inhibition at 250 mM Pseudomonas oleovorans
D-malate important for commercial use, liquid/crystal two phase systems developed to minimize effect Pseudomonas oleovorans
iodoacetamide
-
 Pseudomonas oleovorans
Maleate important for commercial use, liquid/crystal two phase systems developed to minimize effect Pseudomonas oleovorans
Mg2+ 16% inhibition at 250 mM Pseudomonas oleovorans
additional information
-
 Pseudomonas oleovorans

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citraconate
-
 Pseudomonas oleovorans D-citramalate
-
 ?
additional information maleate counterion greatly affects enzyme activity and solute substrate concentration, Ca2+ at 750 mM optimal, followed by Mg2+ Pseudomonas oleovorans ?
-
 ?