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Literature summary for 4.2.1.30 extracted from

  • Forage, R.G.; Foster, M.A.
    Resolution of the coenzyme B-12-dependent dehydratases of Klebsiella sp. and Citrobacter freundii (1979), Biochim. Biophys. Acta, 569, 249-258.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00002
-
 1,2-propanediol coenzyme B12 Klebsiella pneumoniae
0.000021
-
 1,2-propanediol K+ as cofactor Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
 Citrobacter freundii
K+ required Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol Klebsiella pneumoniae
-
 ?
-
 ?
glycerol Citrobacter freundii
-
 ?
-
 ?
additional information Klebsiella pneumoniae glycerol fermentation induces both glycerol dehydratase and diol dehydratase in Klebsiella pneumoniae, ATCC 25955, and in Citrobacter freundii, NCIB 3735 ?
-
 ?
additional information Citrobacter freundii glycerol fermentation induces both glycerol dehydratase and diol dehydratase in Klebsiella pneumoniae, ATCC 25955, and in Citrobacter freundii, NCIB 3735 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Citrobacter freundii
-
 NCIB 3735
-
Klebsiella pneumoniae
-
 ATCC 25955
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Klebsiella pneumoniae
additional information
-
-
 Citrobacter freundii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-ethanediol ethan-1,2-diol Klebsiella pneumoniae acetaldehyde + H2O
-
 ?
1,2-ethanediol ethan-1,2-diol Citrobacter freundii acetaldehyde + H2O
-
 ?
1,2-propanediol propane-1,2-diol Klebsiella pneumoniae propionaldehyde + H2O
-
 ?
1,2-propanediol propane-1,2-diol Citrobacter freundii propionaldehyde + H2O
-
 ?
glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O 3-hydroxypropionaldehyde ?
glycerol
-
 Citrobacter freundii 3-hydroxypropanal + H2O 3-hydroxypropionaldehyde ?
glycerol
-
 Klebsiella pneumoniae ?
-
 ?
glycerol
-
 Citrobacter freundii ?
-
 ?
additional information glycerol fermentation induces both glycerol dehydratase and diol dehydratase in Klebsiella pneumoniae, ATCC 25955, and in Citrobacter freundii, NCIB 3735 Klebsiella pneumoniae ?
-
 ?
additional information glycerol fermentation induces both glycerol dehydratase and diol dehydratase in Klebsiella pneumoniae, ATCC 25955, and in Citrobacter freundii, NCIB 3735 Citrobacter freundii ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
5,6-Dimethylbenzimidazolylcobamide coenzyme required Klebsiella pneumoniae
5,6-Dimethylbenzimidazolylcobamide coenzyme required Citrobacter freundii