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Literature summary for 4.2.1.30 extracted from

  • Stroinski, A.; Pawelkiewicz, J.; Johnson, B.C.
    Allosteric interactions in glycerol dehydratase. Purification of enzyme and effects of positive and negative cooperativity for glycerol (1974), Arch. Biochem. Biophys., 162, 321-330.
    View publication on PubMed
Search for more literature for 4.2.1.30

General Stability

General Stability Organism
glycerol stabilizes the apoenzyme Klebsiella pneumoniae
K2SO3 stabilizes the holoenzyme Klebsiella pneumoniae
stabilization by substrate 1,3-butanediol Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
tetraethylammonium chloride blocks the apoenzyme assay Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required Klebsiella pneumoniae
K+ required for holoenzyme Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 gel filtration, in the presence of ethanolamine buffer containing 0.1 M KCl, subunit B dissociates into sub-subunits Klebsiella pneumoniae
189000
-
 gel filtration, in the presence of ethanolamine buffer, subunit B Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol Klebsiella pneumoniae
-
 ?
-
 ?
glycerol Lactobacillus leichmannii
-
 ?
-
 ?
glycerol Klebsiella pneumoniae PZH 572 Warsaw
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae PZH 572 Warsaw
-
-
-
Lactobacillus leichmannii
-
-
-

Oxidation Stability

Oxidation Stability Organism
under aerobic conditions of incubation holoenzyme is quite unstable and quickly undergoes spontaneous irreversible inactivation Klebsiella pneumoniae

Purification (Commentary)

Purification (Comment) Organism
apoenzyme, AB Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-butanediol
-
 Klebsiella pneumoniae ? + H2O
-
 ?
1,3-butanediol
-
 Klebsiella pneumoniae PZH 572 Warsaw ? + H2O
-
 ?
glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O beta-hydroxypropionaldehyde ?
glycerol
-
 Lactobacillus leichmannii 3-hydroxypropanal + H2O beta-hydroxypropionaldehyde ?
glycerol
-
 Klebsiella pneumoniae PZH 572 Warsaw 3-hydroxypropanal + H2O beta-hydroxypropionaldehyde ?
glycerol
-
 Klebsiella pneumoniae ?
-
 ?
glycerol
-
 Lactobacillus leichmannii ?
-
 ?
glycerol
-
 Klebsiella pneumoniae PZH 572 Warsaw ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 30 min, potassium phosphate buffer, pH 8.6, apoenzyme is quite stable. On addition of coenzyme B12 to the incubation mixture the holoenzyme is formed Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
5,6-Dimethylbenzimidazolylcobamide coenzyme required Klebsiella pneumoniae
5,6-Dimethylbenzimidazolylcobamide coenzyme required Lactobacillus leichmannii