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Literature summary for 4.2.1.30 extracted from

  • Schneider, Z.; Johnson, B.C.
    Studies on the thermal dissociation of glycerol dehydrase (1971), Arch. Biochem. Biophys., 144, 491-495.
    View publication on PubMed
Search for more literature for 4.2.1.30

Inhibitors

Inhibitors Comment Organism Structure
Li+ inactivates subunit B Klebsiella pneumoniae
Na+ inactivates subunit B Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stabilizes subunit A Klebsiella pneumoniae
Na+ 2 M NaCl, stabilizes subunit A Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol Klebsiella pneumoniae
-
 ?
-
 ?
glycerol Klebsiella pneumoniae PZH 572 Warsaw
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae PZH 572 Warsaw
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
 ?
glycerol
-
 Klebsiella pneumoniae PZH 572 Warsaw 3-hydroxypropanal + H2O
-
 ?
glycerol
-
 Klebsiella pneumoniae ?
-
 ?
glycerol
-
 Klebsiella pneumoniae PZH 572 Warsaw ?
-
 ?

Subunits

Subunits Comment Organism
dimer enzyme consists of two different protein subunits, A and B which associate in the presence of K+ or NH4+ forming an active apoenzyme, AB Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 studies on the thermal dissociation of the enzyme Klebsiella pneumoniae
50
-
 subunit A: quickly inactivated at temperatures above, K+ and Na+ stabilize at high concentrations Klebsiella pneumoniae
60
-
 dissociation of the inactive enzyme complex with hydroxocobalamin, if the enzyme complex is heated to over 60°C. Heating products: an active subunit B, an inactive subunit A, and a B12 derivative. K+ inhibit heat-mediated dissociation Klebsiella pneumoniae
75
-
 subunit B is stabilized by K+, Na2HPO4 and K2HPO4 protect against thermal inactivation Klebsiella pneumoniae
83
-
 3 min, 0.6 M KCl, inactive enzyme complex with hydroxocobalamin loses 50% of activity, K+ and Rb+ have the greatest heat-stabilizing effect Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
5,6-Dimethylbenzimidazolylcobamide coenzyme required Klebsiella pneumoniae