Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethyl picolinate | isoenzyme is inhibited, isoenzyme I is less or not sensitive | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
isocitrate | isoenzyme II | Bacillus cereus | |
1.4 | - |
citrate | isoenzyme II | Bacillus cereus | |
3.33 | - |
isocitrate | isoenzyme I | Bacillus cereus | |
10 | - |
citrate | isoenzyme I | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | 80000 | isoenzyme II, gel filtration | Bacillus cereus |
150000 | 160000 | isoenzyme I, gel filtration | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
early aconitase (isoenzyme I) is present in a culture of 5 h age, late aconitase (isoenzyme II) is present in 12 h age | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | isoenzyme I | Bacillus cereus |
30 | - |
isoenzyme II | Bacillus cereus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | about 60% of maximal activity at 20°C and at 40°C, isoenzyme I. 20°C: about 40% of maximal activity, 40°C: about 20% of maximal activity, isoenzyme II | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
isoenzyme II | Bacillus cereus |
7.5 | 8 | isoenzyme I | Bacillus cereus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6: about 55% of maximal activity, pH 9: about 40% of maximal activity, isoenzyme I. pH 6: about 25% of maximal activity, pH 9: about 75% of maximal activity isoenzyme II | Bacillus cereus |