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Literature summary for 4.2.1.28 extracted from

  • Yamanishi, M.; Kinoshita, K.; Fukuoka, M.; Saito, T.; Tanokuchi, A.; Ikeda, Y.; Obayashi, H.; Mori, K.; Shibata, N.; Tobimatsu, T.; Toraya, T.
    Redesign of coenzyme B12 dependent diol dehydratase to be resistant to the mechanism-based inactivation by glycerol and act on longer chain 1,2-diols (2012), FEBS J., 279, 793-804.
    View publication on PubMed
Search for more literature for 4.2.1.28

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Klebsiella oxytoca

Crystallization (Commentary)

Crystallization (Comment) Organism
complexed with cyanocobalamin and glycerol, sandwich-drop vapor diffusion method, using 20 mM Tris/HCl buffer (pH 8.0) containing 14%-18% (w/v) poly(ethylene glycol) 6000, 0.23-0.25 M ammonium sulfate and 0.2% lauryl dimethylamine oxide Klebsiella oxytoca

Protein Variants

Protein Variants Comment Organism
Q336A the mutant shows decreased activity (21%) compared to the wild type enzyme Klebsiella oxytoca
S301A the mutant shows decreased activity (76%) compared to the wild type enzyme Klebsiella oxytoca
S301A/Q336A the mutant shows lowest activity (9.8%) compared to the wild type enzyme Klebsiella oxytoca

Inhibitors

Inhibitors Comment Organism Structure
glycerol
-
 Klebsiella oxytoca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 (S)-propane-1,2-diol wild type enzyme, at pH 8.0 and 37°C Klebsiella oxytoca
0.26
-
 (S)-propane-1,2-diol mutant enzyme S301A, at pH 8.0 and 37°C Klebsiella oxytoca
0.47
-
 (R)-Propane-1,2-diol wild type enzyme, at pH 8.0 and 37°C Klebsiella oxytoca
0.66
-
 (S)-propane-1,2-diol mutant enzyme Q336A, at pH 8.0 and 37°C Klebsiella oxytoca
0.7
-
 (R)-Propane-1,2-diol mutant enzyme S301A, at pH 8.0 and 37°C Klebsiella oxytoca
2.1
-
 (R)-Propane-1,2-diol mutant enzyme Q336A, at pH 8.0 and 37°C Klebsiella oxytoca
2.6
-
 (S)-propane-1,2-diol mutant enzyme S301A/Q336A, at pH 8.0 and 37°C Klebsiella oxytoca
3.9
-
 (R)-Propane-1,2-diol mutant enzyme S301A/Q336A, at pH 8.0 and 37°C Klebsiella oxytoca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-propane-1,2-diol Klebsiella oxytoca
-
 propanal + H2O
-
 ?
(R)-propane-1,2-diol Klebsiella oxytoca ATCC 8724
-
 propanal + H2O
-
 ?
(S)-propane-1,2-diol Klebsiella oxytoca
-
 propanal + H2O
-
 ?
(S)-propane-1,2-diol Klebsiella oxytoca ATCC 8724
-
 propanal + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella oxytoca Q59470
-
-
Klebsiella oxytoca ATCC 8724 Q59470
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography Klebsiella oxytoca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-propane-1,2-diol
-
 Klebsiella oxytoca propanal + H2O
-
 ?
(R)-propane-1,2-diol
-
 Klebsiella oxytoca ATCC 8724 propanal + H2O
-
 ?
(S)-propane-1,2-diol
-
 Klebsiella oxytoca propanal + H2O
-
 ?
(S)-propane-1,2-diol
-
 Klebsiella oxytoca ATCC 8724 propanal + H2O
-
 ?
butane-1,2-diol 107% activity compared to propane-1,2-diol Klebsiella oxytoca ?
-
 ?
butane-1,2-diol 107% activity compared to propane-1,2-diol Klebsiella oxytoca ATCC 8724 ?
-
 ?
additional information no activity with hexane-1,2-diol Klebsiella oxytoca ?
-
 ?
additional information no activity with hexane-1,2-diol Klebsiella oxytoca ATCC 8724 ?
-
 ?

Synonyms

Synonyms Comment Organism
diol dehydratase alpha subunit
-
 Klebsiella oxytoca

Cofactor

Cofactor Comment Organism Structure
coenzyme B12 dependent on Klebsiella oxytoca