Literature summary for 4.2.1.28 extracted from

Kawata, M.; Kinoshita, K.; Takahashi, S.; Ogura, K.; Komoto, N.; Yamanishi, M.; Tobimatsu, T.; Toraya, T.
Survey of Catalytic Residues and Essential Roles of Glutamate-aplpha170 and Aspartate-alpha335 in Coenzyme B12-dependent Diol Dehydratase (2006), J. Biol. Chem., 281, 18327-18334.

Search for more literature for 4.2.1.28

Crystallization (Commentary)

Crystallization (Comment)	Organism
as complexes with coenzyme analogs in the substrate-bound and substrate-free forms	Klebsiella oxytoca

Protein Variants

Protein Variants	Comment	Organism
D335A	inactive mutant	Klebsiella oxytoca
D335A	does not form (alpha-beta-gamma)complex	Klebsiella oxytoca
D335N	inactive mutant	Klebsiella oxytoca
E170A	inactive mutant	Klebsiella oxytoca
E170H	does not form (alpha-beta-gamma)complex	Klebsiella oxytoca
E221A	does not form (alpha-beta-gamma)complex	Klebsiella oxytoca
H143A	only very little activity	Klebsiella oxytoca
Q141A	partial activity	Klebsiella oxytoca
Q296A	increased Km for substrate(1,2-propanediol) by a factor of 250	Klebsiella oxytoca
S362A	partial activity	Klebsiella oxytoca

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	1,2-propanediol	-	Klebsiella oxytoca
0.08	-	1,2-propanediol	mutant S362A	Klebsiella oxytoca
0.1	-	1,2-propanediol	mutant Q141A	Klebsiella oxytoca
10	-	1,2-propanediol	mutant Q296A	Klebsiella oxytoca

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	-	Klebsiella oxytoca

Organism

Organism	UniProt	Comment	Textmining
Klebsiella oxytoca	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Klebsiella oxytoca

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,2-ethanediol	-	Klebsiella oxytoca	acetaldehyde + H2O	-	?
1,2-propanediol	-	Klebsiella oxytoca	propanal + H2O	-	?
glycerol	-	Klebsiella oxytoca	beta-hydroxypropionaldehyde + H2O	-	?

Subunits

Subunits	Comment	Organism
hexamer	dimer of a heterotrimer	Klebsiella oxytoca

Synonyms

Synonyms	Comment	Organism
diol dehydratase	-	Klebsiella oxytoca

Cofactor

Cofactor	Comment	Organism	Structure
adenosylcobalamin	-	Klebsiella oxytoca

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Release 2023.1 (January 2023)