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Literature summary for 4.2.1.28 extracted from
- Diol dehydratase: N-terminal amino acid sequences and subunit stoichiometry (1982), Biochem. Biophys. Res. Commun., 108, 547-551.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell membrane | peripheral membrane protein | Klebsiella pneumoniae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | - |
Klebsiella pneumoniae |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
2 * 60000, 1 * 51000, 2 * 29000, 2 * 15000, SDS-PAGE, subunit stoichiometry confirmed by N-terminal amino acid analysis | Klebsiella pneumoniae |
| 29000 | - |
2 * 60000, 1 * 51000, 2 * 29000, 2 * 15000, SDS-PAGE, subunit stoichiometry confirmed by N-terminal amino acid analysis | Klebsiella pneumoniae |
| 51000 | - |
2 * 60000, 1 * 51000, 2 * 29000, 2 * 15000, SDS-PAGE, subunit stoichiometry confirmed by N-terminal amino acid analysis | Klebsiella pneumoniae |
| 60000 | - |
2 * 60000, 1 * 51000, 2 * 29000, 2 * 15000, SDS-PAGE, subunit stoichiometry confirmed by N-terminal amino acid analysis | Klebsiella pneumoniae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 82.2 | - |
- |
Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-propanediol | - |
Klebsiella pneumoniae | propanal | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heptamer | 2 * 60000, 1 * 51000, 2 * 29000, 2 * 15000, SDS-PAGE, subunit stoichiometry confirmed by N-terminal amino acid analysis | Klebsiella pneumoniae |
| More | role of 60000 and 29000 subunits as membrane anchors, role of 51000 subunit for coenzyme binding | Klebsiella pneumoniae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cobamide | - |
Klebsiella pneumoniae | |
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Release 2023.1 (January 2023)
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