Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.24 extracted from

  • Cheung, K.M.; Spencer, P.; Timko, M.P.; Shoolingin-Jordan, P.M.
    Characterization of a recombinant pea 5-aminolevulinic acid dehydratase and comparative inhibition studies with the Escherichia coli dehydratase (1997), Biochemistry, 36, 1148-1156.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pisum sativum

Inhibitors

Inhibitors Comment Organism Structure
3-acetyl-4-oxoheptane-1,7-dioic acid formation of a Schiff base complex between the inhibitors and the active site Lys Pisum sativum
4-amino-3-oxobutanoate
-
Pisum sativum
Co2+ above 1 mM Pisum sativum
Mn2+ above 1 mM Pisum sativum
phosphate competitive against Mg2+ Pisum sativum
succinylacetone
-
Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
5-aminolevulinate at pH 7.5 Pisum sativum
1
-
5-aminolevulinate at pH 9 Pisum sativum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Pisum sativum
Co2+ about 50% reactivation of the demetalled protein Pisum sativum
Mg2+ required Pisum sativum
Mg2+ activates Pisum sativum
Mg2+ 2 Mg2+ binding sites per subunit Pisum sativum
Mn2+ activates Pisum sativum
Mn2+ can reactivate the demetallated protein Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
8 * 43000, SDS-PAGE Pisum sativum
309000
-
gel filtration Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pisum sativum

Reaction

Reaction Comment Organism Reaction ID
2 5-aminolevulinate = porphobilinogen + 2 H2O substrate is covalently bound through a Schiff base Pisum sativum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.33
-
-
Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-aminolevulinate + 5-aminolevulinate
-
Pisum sativum porphobilinogen + 2 H2O
-
?

Subunits

Subunits Comment Organism
octamer 8 * 43000, SDS-PAGE Pisum sativum