Activating Compound | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | structure of the regulatory, energy-sensing CBS domains and mechanism for allosteric activation by S-adenoyl-L-methionine, overview | Drosophila melanogaster |
Crystallization (Comment) | Organism |
---|---|
enzyme free or in complex with a carbanion and an aminoacrylate intermediate, X-ray diffraction structure determination and analysis at 1.70 A and 1.55 A resolution, respectively | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + L-homocysteine | Drosophila melanogaster | - |
L-cystathionine + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9VRD9 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-serine + L-homocysteine = L-cystathionine + H2O | carbanion and aminacrylate intermediates in the CBS-catalyzed reaction, arrangement of the protein residues and pyridoxal 5'-phosphate cofactor in the active site pocket, and reaction mechanism, overview | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + L-homocysteine | - |
Drosophila melanogaster | L-cystathionine + H2O | - |
? | |
L-serine + L-homocysteine | electrostatic stabilization of the zwitterionic carbanion intermediate is afforded by the close positioning of an active site lysine residue that is initially used for Schiff base formation in the internal aldimine and later as a general base, and additional stabilizing interactions between active site residues and the catalytic intermediates | Drosophila melanogaster | L-cystathionine + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CBS | - |
Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
additional information | structural basis for substrate activation and regulation by cystathionine beta-synthase domains in cystathionine beta-synthase, allosteric regulation via the CBS domains, mechanism, overview | Drosophila melanogaster |