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Literature summary for 4.2.1.22 extracted from

  • Koutmos, M.; Kabil, O.; Smith, J.L.; Banerjee, R.
    Structural basis for substrate activation and regulation by cystathionine beta-synthase (CBS) domains in cystathionine beta-synthase (2010), Proc. Natl. Acad. Sci. USA, 107, 20958-20963.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
S-adenosyl-L-methionine structure of the regulatory, energy-sensing CBS domains and mechanism for allosteric activation by S-adenoyl-L-methionine, overview Drosophila melanogaster

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme free or in complex with a carbanion and an aminoacrylate intermediate, X-ray diffraction structure determination and analysis at 1.70 A and 1.55 A resolution, respectively Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + L-homocysteine Drosophila melanogaster
-
L-cystathionine + H2O
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q9VRD9
-
-

Reaction

Reaction Comment Organism Reaction ID
L-serine + L-homocysteine = L-cystathionine + H2O carbanion and aminacrylate intermediates in the CBS-catalyzed reaction, arrangement of the protein residues and pyridoxal 5'-phosphate cofactor in the active site pocket, and reaction mechanism, overview Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + L-homocysteine
-
Drosophila melanogaster L-cystathionine + H2O
-
?
L-serine + L-homocysteine electrostatic stabilization of the zwitterionic carbanion intermediate is afforded by the close positioning of an active site lysine residue that is initially used for Schiff base formation in the internal aldimine and later as a general base, and additional stabilizing interactions between active site residues and the catalytic intermediates Drosophila melanogaster L-cystathionine + H2O
-
?

Synonyms

Synonyms Comment Organism
CBS
-
Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Drosophila melanogaster

General Information

General Information Comment Organism
additional information structural basis for substrate activation and regulation by cystathionine beta-synthase domains in cystathionine beta-synthase, allosteric regulation via the CBS domains, mechanism, overview Drosophila melanogaster