Activating Compound | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | allosteric activator | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and Co-subsituted CBS as GST-tagged enzymes in Escherichia coli strain Rosetta 2 (DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a cobalt CBS, CoCBS, by metalloporphyrin replacement, which results in a high yield of fully active, high purity enzyme, in which heme is substituted by Co-protoporphyrin IX, CoPPIX. The enzyme contains 92% cobalt and 8% iron. CoCBS is indistinguishable from wild-type FeCBS in its activity, tetrameric oligomerization, PLP saturation and responsiveness to the allosteric activator, S-adenosyl-L-methionine | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 97.1% Fe2+ and 2.9% Co2+ in wild-type FeCBS enzyme, 8% Fe2+ and 92% Co2+ in the CoCBS enzyme variant | Homo sapiens | |
Fe2+ | heme enzyme, 97.1% Fe2+ and 2.9% Co2+ in wild-type FeCBS enzyme, 8% Fe2+ and 92% Co2+ in the CoCBS enzyme variant | Homo sapiens | |
additional information | construction of a cobalt CBS, CoCBS, by metalloporphyrin replacement, which results in a high yield of fully active, high purity enzyme, in which heme is substituted by Co-protoporphyrin IX, CoPPIX. the enzyme contains 92% cobalt and 8% iron. CoCBS is indistinguishable from wild-type FeCBS in its activity, tetrameric oligomerization, PLP saturation and responsiveness to the allosteric activator, S-adenosyl-L-methionine | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
4 * 63000 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged wild-type and Co-subsituted CBS from Escherichia coli strain Rosetta 2 (DE3) by glutathione affinity and anion exchange chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
cystathionine synthesis activity of FeCBS and CoCBS | Homo sapiens |
105.2 | - |
purified recombinant CoCBS, pH 8.6, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme performs L-cysteine sythesis from L-serine | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 63000 | Homo sapiens |
More | each subunit has a modular structure consisting of three domains: an N-terminal heme binding domain, a highly conserved pyridoxal 5'-phosphate-binding catalytic core, and a S-adenosyl-L-methionine-binding C-terminal regulatory domain | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CBS | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Homo sapiens | |
pyridoxal 5'-phosphate | i.e. PLP, serves in the catalytic chemistry of CBS via a well-established mechanism | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | cystathionine beta-synthase is a pivotal enzyme in the metabolism of homocysteine, and is a pyridoxal 5'-phosphate-dependent enzyme that also contains heme as a second cofactor | Homo sapiens |