Literature summary for 4.2.1.22 extracted from

Celano, L.; Gil, M.; Carballal, S.; Duran, R.; Denicola, A.; Banerjee, R.; Alvarez, B.
Inactivation of cystathionine beta-synthase with peroxynitrite (2009), Arch. Biochem. Biophys., 491, 96-105.

Search for more literature for 4.2.1.22

Cloned(Commentary)

Cloned (Comment)	Organism
truncated human CBS lacking 143 amino acids at the C-terminus is expressed as a fusion protein with glutathione S-transferase in the Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
DELTAC143	a truncated human CBS lacking 143 amino acids at the C-terminus is used to study the inactivation of cystathionine beta-synthase by peroxynitrite	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
peroxynitrite	exposure to peroxynitrite does not modify bound pyridoxal 5'-phosphate but leads to nitration of Trp208, Trp43 and Tyr223 and alterations in the heme environment including loss of thiolate coordination, conversion to high-spin and bleaching, with no detectable formation of oxoferryl compounds nor promotion of one-electron processes	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe3+	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	the protein is an active and stable dimer of 45 kDa subunits	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P35520	-	-

Purification (Commentary)

Purification (Comment)	Organism
truncated human CBS lacking 143 amino acids at the C-terminus is purified as a fusion protein with glutathione S-transferase using the Escherichia coli expression vector pGEXCBSN. The protein is purified through affinity chromatography with glutathione sepharose and anion exchange chromatography. The glutathione Stransferase tag was cleaved by limited proteolysis using thrombin	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
9.8	-	-	Homo sapiens

Subunits

Subunits	Comment	Organism
dimer	2 * 45000 Da, truncated human CBS lacking 143 amino acids at the C-terminus	Homo sapiens

Synonyms

Synonyms	Comment	Organism
CBS	-	Homo sapiens
cystathionine beta-synthase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.3	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Homo sapiens
pyridoxal 5'-phosphate	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.15	-	-	Homo sapiens	peroxynitrite

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Release 2023.1 (January 2023)