Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain DH10B | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-homocysteine | substrate inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
L-cystathionine | recombinant 6-His-tagged enzyme, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens | |
0.43 | - |
L-homocysteine | recombinant 6-His-tagged enzyme, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens | |
5 | - |
L-serine | recombinant 6-His-tagged enzyme, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
His-tagged enzyme, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.045 | - |
recombinant 6-His-tagged enzyme from crude extract, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens |
3.17 | - |
recombinant 6-His-tagged enzyme after purification, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + L-homocysteine | - |
Homo sapiens | L-cystathionine + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CBS | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
L-homocysteine | recombinant 6-His-tagged enzyme, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens | |
1.67 | - |
L-serine | recombinant 6-His-tagged enzyme, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
around pH 8.6 | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Homo sapiens | |
pyridoxal 5'-phosphate | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
L-homocysteine | recombinant 6-His-tagged enzyme, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens |