Activating Compound | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | stimulates | Homo sapiens | |
S-adenosylhomocysteine | stimulates activity 1.1fold at 0.48 mM | Homo sapiens | |
sinefungin | stimulates activity 1.28fold at 1 mM | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of wild type and truncated human cystathionine beta-synthase enzyme in Escherichia coli | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
L-homocysteine | 37°C, pH 8.0, wild-type enzyme, with S-adenosyl-L-methionine | Homo sapiens | |
1.04 | - |
L-homocysteine | 37°C, pH 8.0, wild-type enzyme, without S-adenosyl-L-methionine | Homo sapiens | |
1.41 | - |
L-serine | 37°C, pH 8.0, wild-type enzyme, without -adenosyl-L-methionine | Homo sapiens | |
2.13 | - |
L-serine | 37°C, pH 8.0, wild-type enzyme, with S-adenosyl-L-methionine | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild type and truncated human cystathionine beta-synthase expressed in Escherichia coli | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.3 | - |
wild-type enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + L-homocysteine | - |
Homo sapiens | cystathionine + H2O | - |
? | |
O-acetyl-L-serine + L-homocysteine | poor substrate for both the wild type and the N- and C-terminally truncated enzyme 71400 CBS | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
L-serine | 37°C, pH 8.0, wild-type enzyme, with S-adenosyl-L-methionine | Homo sapiens | |
3.3 | - |
L-homocysteine | 37°C, pH 8.0, wild-type enzyme, without S-adenosyl-L-methionine | Homo sapiens | |
3.67 | - |
L-serine | 37°C, pH 8.0, wild-type enzyme, without S-adenosyl-L-methionine | Homo sapiens | |
4.66 | - |
L-homocysteine | 37°C, pH 8.0, wild-type enzyme, without S-adenosyl-L-methionine | Homo sapiens | |
12.7 | - |
L-homocysteine | 37°C, pH 8.0, wild-type enzyme, with S-adenosyl-L-methionine | Homo sapiens | |
14.01 | - |
L-serine | 37°C, pH 8.0, wild-type enzyme, with S-adenosyl-L-methionine | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
broad, full-length and the C-terminally truncated enzyme (CBS 1-413) | Homo sapiens |
9 | - |
sharp, N- and C-terminally truncated enzyme (BS 71-400) | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.3 | 9.5 | pH 7.3: about 45% of maximal activity, pH 9.5: about 80% of maximal activity, full-length and the C-terminally truncated enzyme (CBS 1-413) | Homo sapiens |
8.3 | 9.5 | pH 8.3: about 45% of maximal activity, pH 9.5: about 85% of maximal activity N- and C-terminally truncated enzyme (BS 71-400) | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | cystathionine beta-synthase binds one mole of heme per subunit | Homo sapiens |