Literature summary for 4.2.1.20 extracted from

Phillips, R.S.; Wang, A.K.; Marchal, S.; Lange, R.
Effects of pressure and osmolytes on the allosteric equilibria of Salmonella typhimurium tryptophan synthase (2012), Biochemistry, 51, 9354-9363.

Search for more literature for 4.2.1.20

Activating Compound

Activating Compound	Comment	Organism	Structure
betaine	1 M betaine activates the enzyme 1.8fold in the absence of monovalent cations	Salmonella enterica subsp. enterica serovar Typhimurium
polyethylene glycol 8000	5% (w/v) activates the enzyme 2.1fold in the absence of monovalent cations	Salmonella enterica subsp. enterica serovar Typhimurium
sucrose	2 M sucrose activates the enzyme 3.3fold in the absence of monovalent cations	Salmonella enterica subsp. enterica serovar Typhimurium
taurine	0.5 M taurine activates the enzyme 3fold in the absence of monovalent cations	Salmonella enterica subsp. enterica serovar Typhimurium

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Salmonella enterica subsp. enterica serovar Typhimurium

General Stability

General Stability	Organism
in the presence of monovalent cations, osmolytes have only minor effects on activity and allosteric equilibria, but 1 M betaine stabilizes the Trp synthase-L-Ser-indoline complex against apparent pressure-induced subunit dissociation	Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	activates at 0.1 M	Salmonella enterica subsp. enterica serovar Typhimurium
Na+	activates at 0.1 M	Salmonella enterica subsp. enterica serovar Typhimurium
NH4+	activates at 0.1 M	Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium	-	L-tryptophan + D-glyceraldehyde 3-phosphate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium	L-tryptophan + D-glyceraldehyde 3-phosphate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
Trp synthase	-	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)