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Literature summary for 4.2.1.20 extracted from

  • Shen, H.; Yang, Y.; Wang, F.; Zhang, Y.; Ye, N.; Xu, S.; Wang, H.
    Characterization of the putative tryptophan synthase beta-subunit from Mycobacterium tuberculosis (2009), Acta Biochim. Biophys. Sin., 41, 379-388.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
over-expressed in Mycobacterium tuberculosis H37Rv as a His-tagged fusion protein Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
H100A Km (indole), (L-serine) higher than wild-type, kcat (indole), (L-serine) significantly decreased compared to wild-type Mycobacterium tuberculosis
N250A Km (indole), (L-serine) higher than wild-type, kcat (indole), (L-serine) lower compared to wild-type Mycobacterium tuberculosis
Q128A Km (indole), (L-serine) higher than wild-type, kcat (indole) slightly lower than wild-type, kcat (L-serine) lower compared to wild-type Mycobacterium tuberculosis
Q364A Km (indole), (L-serine) higher than wild-type, kcat (indole), (L-serine) significantly decreased compared to wild-type Mycobacterium tuberculosis
S249A Km (indole), (L-serine) higher than wild-type, kcat (indole), (L-serine) lower compared to wild-type Mycobacterium tuberculosis
S390A Km (indole), (L-serine) higher than wild-type, kcat (indole), (L-serine) lower compared to wild-type Mycobacterium tuberculosis
S99A Km (indole), (L-serine) higher than wild-type, kcat (indole), (L-serine) lower compared to wild-type Mycobacterium tuberculosis
T204A Km (indole), (L-serine) higher than wild-type, kcat (indole), (L-serine) lower compared to wild-type Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.178
-
L-serine
-
Mycobacterium tuberculosis
0.288
-
indole
-
Mycobacterium tuberculosis
0.983
-
L-serine mutant T204A Mycobacterium tuberculosis
0.997
-
indole mutant Q128A Mycobacterium tuberculosis
1.013
-
indole mutant T204A Mycobacterium tuberculosis
1.052
-
L-serine mutant Q128A Mycobacterium tuberculosis
1.235
-
L-serine mutant S249A Mycobacterium tuberculosis
1.485
-
indole mutant S249A Mycobacterium tuberculosis
1.683
-
L-serine mutant S99A Mycobacterium tuberculosis
1.85
-
L-serine mutant N250A Mycobacterium tuberculosis
1.911
-
L-serine mutant S390A Mycobacterium tuberculosis
2.083
-
indole mutant S390A Mycobacterium tuberculosis
2.342
-
indole mutant S99A Mycobacterium tuberculosis
2.86
-
indole mutant N250A Mycobacterium tuberculosis
2.906
-
indole mutant Q364A Mycobacterium tuberculosis
4
-
L-serine mutant Q364A Mycobacterium tuberculosis
4.471
-
indole mutant H100A Mycobacterium tuberculosis
15.8
-
L-serine mutant H100A Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ highest activity with 0.18 M Mg2+ Mycobacterium tuberculosis
Na+ highest activity with 0.15 M Na+ Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
SDS-PAGE, recombinant protein Mycobacterium tuberculosis
50220
-
mass spectroscopy Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using metal affinity chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
indole + L-serine
-
Mycobacterium tuberculosis L-tryptophan + H2O
-
?

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
beta subunit of tryptophan synthase
-
Mycobacterium tuberculosis
Rv1612
-
Mycobacterium tuberculosis
TrB
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 80 the enzyme activity decreases significantly when the temperature is above 40°C. The activity of enzyme incubated at 80°C is below 10% of that incubated at 10°C Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.987
-
L-serine mutant H100A Mycobacterium tuberculosis
2.531
-
indole mutant H100A Mycobacterium tuberculosis
3.462
-
L-serine mutant Q364A Mycobacterium tuberculosis
6.427
-
indole mutant Q364A Mycobacterium tuberculosis
7.106
-
L-serine mutant N250A Mycobacterium tuberculosis
10.69
-
L-serine mutant S249A Mycobacterium tuberculosis
13.22
-
L-serine mutant S99A Mycobacterium tuberculosis
16.35
-
L-serine mutant T204A Mycobacterium tuberculosis
20.48
-
L-serine mutant S390A Mycobacterium tuberculosis
21.14
-
indole mutant S99A Mycobacterium tuberculosis
21.47
-
L-serine mutant Q128A Mycobacterium tuberculosis
26.84
-
indole mutant S249A Mycobacterium tuberculosis
34.73
-
indole mutant S390A Mycobacterium tuberculosis
40.18
-
indole mutant N250A Mycobacterium tuberculosis
48.21
-
L-serine
-
Mycobacterium tuberculosis
50.65
-
indole mutant T204A Mycobacterium tuberculosis
68.43
-
indole mutant Q128A Mycobacterium tuberculosis
70.29
-
indole
-
Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Mycobacterium tuberculosis

General Information

General Information Comment Organism
physiological function structure analysis indicate that Mycobacterium tuberculosis TrpB exhibits a typical beta/alpha barrel structure Mycobacterium tuberculosis