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Literature summary for 4.2.1.20 extracted from

  • Ogasahara, K.; Ishida, M.; Yutani, K.
    Stimulated interaction between and subunits of tryptophan synthase from hyperthermophile enhances its thermal stability (2003), J. Biol. Chem., 278, 8922-8928.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genes trpA encoding the alpha-subunit and trpB encoding the beta-subunit are coexpressed or expressed individually in Escherichia coli strain JM109 Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information thermodynamic parameters of subunit association, kinetics Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27500
-
alpha-subunit monomer, ultracentrifugation sedimentation equilibrium Pyrococcus furiosus
42500
-
2 * 275000 + 2 * 42500, recombinant alpha2beta2 enzyme complex, SDS-PAGE Pyrococcus furiosus
42500
-
2 * 42500, recombinant beta-subunit, SDS-PAGE Pyrococcus furiosus
84000 88000 beta-subunit dimer, ultracentrifugation sedimentation equilibrium Pyrococcus furiosus
140000
-
enzyme complex alpha2beta2, ultracentrifugation sedimentation equilibrium Pyrococcus furiosus
275000
-
2 * 275000 + 2 * 42500, recombinant alpha2beta2 enzyme complex, SDS-PAGE Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant alpha-subunit, beta-subunit, and enzyme complex expressed in Escherichia coli Pyrococcus furiosus

Reaction

Reaction Comment Organism Reaction ID
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(indol-3-yl)glycerol 3-phosphate alpha-subunit of the bienzyme complex, alpha-reaction Pyrococcus furiosus D-glyceraldehyde 3-phosphate + indole
-
?
L-serine + indole beta-subunit of the bienzyme complex, beta-reaction Pyrococcus furiosus L-tryptophan + H2O
-
?

Subunits

Subunits Comment Organism
dimer 2 * 42500, recombinant beta-subunit, SDS-PAGE Pyrococcus furiosus
monomer 1 * 27500, recombinant alpha-subunit, SDS-PAGE Pyrococcus furiosus
More high association constant for the hyperthermophile enzyme subunits, pH-dependence of the molecular weights Pyrococcus furiosus
tetramer 2 * 275000 + 2 * 42500, recombinant alpha2beta2 enzyme complex, SDS-PAGE Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
inactivation temperature of the alpha-subunit is enhanced by the formation of the enzyme complex alpha2beta2 by 17°C Pyrococcus furiosus