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Literature summary for 4.2.1.20 extracted from

  • Weyand, M.; Schlichting, I.
    Structural basis for the impaired channeling and allosteric inter-subunit communication in the beta A169L/beta C170W mutant of tryptophan synthase (2000), J. Biol. Chem., 275, 41058-41063.
    View publication on PubMed
Search for more literature for 4.2.1.20

Crystallization (Commentary)

Crystallization (Comment) Organism
purified mutant A169L/C170W complexed with the alpha-active site substrate analogue 5-fluoro-indole-propanol-phosphate, X-ray diffraction structure determination and analysis at 2.25 A resolution Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants Comment Organism
A169L/C170W mutants of beta-subunit residues, altered kinetic properties and conformation Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of wild-type enzyme complex and mutant betaA169L/betaC170W Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), mechanism Salmonella enterica subsp. enterica serovar Typhimurium
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(indol-3-yl)glycerol 3-phosphate alpha-subunit of the bifunctional enzyme complex, alpha-reaction Salmonella enterica subsp. enterica serovar Typhimurium D-glyceraldehyde 3-phosphate + indole
-
 ?
L-serine + indole beta-subunit of the bifunctional enzyme complex, beta-reaction Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
-
 ?
additional information substrate channeling, Thr170 occludes the tunnel connecting the alpha- and beta-active sites, accumulation of the intermediate indole during a single enzyme turnover Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha2beta2 complex, structural organization Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms Comment Organism
TRPS
-
 Salmonella enterica subsp. enterica serovar Typhimurium