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Literature summary for 4.2.1.20 extracted from

  • Ro, H.S.; Wilson Miles, E.
    Catalytic mechanism of the tryptophan synthase alpha(2)beta(2) complex. Effects of pH, isotopic substitution, and allosteric ligands (1999), J. Biol. Chem., 274, 31189-31194.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
glycine competitive Salmonella enterica subsp. enterica serovar Typhimurium
additional information inhibition mechanism Salmonella enterica subsp. enterica serovar Typhimurium
oxindolyl-L-alanine competitive Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, temperature-, and pH-dependence, and isotope effect at pH 7.0, not at pH 9.0, overview Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), catalytic mechanism, allosteric ligand binding Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(indol-3-yl)glycerol 3-phosphate + L-serine
-
Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + D-glyceraldehyde 3-phosphate
-
?
serine + indole
-
Salmonella enterica subsp. enterica serovar Typhimurium tryptophan + H2O
-
?

Subunits

Subunits Comment Organism
tetramer alpha2beta2 complex Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.3
-
glycine
-
Salmonella enterica subsp. enterica serovar Typhimurium
7.3
-
oxindolyl-L-alanine
-
Salmonella enterica subsp. enterica serovar Typhimurium