Protein Variants | Comment | Organism |
---|---|---|
D305N | site-directed mutagenesis, residue is involved in formation of salt bridges between the different subunits, kinetic and structural, conformational alterations, altered signaling by ligand binding, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
D56A | site-directed mutagenesis, residue is involved in formation of salt bridges between the different subunits, kinetic and structural, conformational alterations, altered signaling by ligand binding, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
K167T | site-directed mutagenesis, residue is involved in formation of salt bridges between the different subunits, kinetic and structural, conformational alterations, altered signaling by ligand binding, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-glycerol 3-phosphate | inhibits beta-reaction activity of wild-type and mutant D56A | Salmonella enterica subsp. enterica serovar Typhimurium | |
K+ | inhibits mutant D56A beta-reaction activity, inhibits alpha-reaction of all wild-type and mutants, enhances the overall enzyme complex activity for all wild-type and mutant enzymes, except for mutant D56A | Salmonella enterica subsp. enterica serovar Typhimurium | |
Na+ | inhibits beta-reaction activity of mutant D56A, D305N and K167T, inhibits alpha-reaction of wild-type and mutant D56A, enhances the overall enzyme complex activity for all wild-type and mutant enzymes except for mutant D56A | Salmonella enterica subsp. enterica serovar Typhimurium | |
NH4+ | inhibits mutant D56A, D305N and K167T alpha-reaction activity, inhibits alpha-reaction of wild-type and mutant D56A, enhances the overall enzyme complex activity for all wild-type and mutant enzymes | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, wild-type and mutants, isotope effects and effector influence | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
DL-glycerol 3-phosphate | enhances beta-reaction activity of mutant D305N and K167T | Salmonella enterica subsp. enterica serovar Typhimurium | |
K+ | enhances beta-reaction activity of wild-type and mutants, except for mutant D56A | Salmonella enterica subsp. enterica serovar Typhimurium | |
Na+ | enhances beta-reaction activity of wild-type | Salmonella enterica subsp. enterica serovar Typhimurium | |
NH4+ | enhances beta-reaction activity of all wild-type and mutants | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), catalytic mechanism | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | D-glyceraldehyde 3-phosphate + indole | - |
? | |
L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + H2O | - |
? | |
additional information | allostery and substrate channeling, synergism between binding interactions at the monovalent cation binding site, the formation of salt bridges to support allosteric communication between sites, ligand binding to the alpha-site, and the catalytic activities of the alpha- and beta-sites | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure models | Salmonella enterica subsp. enterica serovar Typhimurium |
tetramer | alpha2beta2 enzyme complex | Salmonella enterica subsp. enterica serovar Typhimurium |