Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.20 extracted from

  • Jeong, J.K.; Shin, H.J.; Kim, J.W.; Lee, C.H.; Kim, H.D.; Lim, W.K.
    Fluorescence and folding properties of Tyr mutant tryptophan synthase alpha-subunits from Escherichia coli (2003), Biochem. Biophys. Res. Commun., 300, 29-35.
    View publication on PubMed
Search for more literature for 4.2.1.20

Protein Variants

Protein Variants Comment Organism
Y173F oligonucleotide-directed mutagenesis, gene trpA, alpha-subunit residue exchange, altered fluorescence and folding properties Escherichia coli
Y175F oligonucleotide-directed mutagenesis, gene trpA, alpha-subunit residue exchange, altered fluorescence and folding properties Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) Escherichia coli
a

Renatured (Commentary)

Renatured (Comment) Organism
unfolding and refolding of wild-type and mutant alpha-subunits using urea, comparison of the folding process differences, thermodynamic parameters Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(indol-3-yl)glycerol 3-phosphate alpha-subunit of the bienzyme complex, alpha-reaction Escherichia coli D-glyceraldehyde 3-phosphate + indole
-
 ?
L-serine + indole beta-subunit of the bienzyme complex, beta-reaction Escherichia coli L-tryptophan + H2O
-
 ?

Synonyms

Synonyms Comment Organism
alphaTS
-
 Escherichia coli