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Literature summary for 4.2.1.2 extracted from

  • Rose, I.A.; Weaver, T.M.
    The role of the allosteric B site in the fumarase reaction (2004), Proc. Natl. Acad. Sci. USA, 101, 3393-3397.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.2

Protein Variants

Protein Variants Comment Organism
H129N loss of D2O inhibitory effect, product release step is accelerated by glycerol compared to inhibition of wild-type enzyme. 3.1fold reduced maximal velococity in reaction with malate, 1.13fold reduced maximal velocity in reaction with fumarate compared to wild-type enzyme Escherichia coli
K127D mutant enzyme behaves like wild-type enzyme Escherichia coli
R126A loss of D2O inhibitory effect, product release step is accelerated by glycerol compared to inhibition of wild-type enzyme. 4.3fold reduced maximal velococity in reaction with malate, 2.7fold reduced maximal velocity in reaction with fumarate compared to wild-type enzyme Escherichia coli
R126A/H129N great loss of D2O inhibitory effect, product release step is accelerated by glycerol compared to inhibition of wild-type enzyme. 8.6fold reduced maximal velococity in reaction with malate, 7.1fold reduced maximal velocity in reaction with fumarate compared to wild-type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
glycerol wild-type enzyme is inhibited due to a viscogenic effect on the recycling rate Escherichia coli
meso-tartrate
-
 Escherichia coli
additional information inhibition by D2O arises in the recycling phase Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 FumC
-
Escherichia coli FumC
-
 FumC
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate
-
 Escherichia coli fumarate + H2O wild-type enzyme is rate-limited in the recycling of free enzyme isoforms that follows product release ?
(S)-malate
-
 Escherichia coli FumC fumarate + H2O wild-type enzyme is rate-limited in the recycling of free enzyme isoforms that follows product release ?

Synonyms

Synonyms Comment Organism
FumC
-
 Escherichia coli