General Stability | Organism |
---|---|
exposure to air at room temperature causes 50% loss of activity, reactivation with FeSO4 and 2-mercaptoethanol | Bacteria |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
fumarate | - |
Bacteria | |
2.38 | - |
L-malate | - |
Bacteria |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 * 60000, SDS-PAGE | Bacteria |
114000 | - |
gel filtration | Bacteria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteria | - |
synthrophic propionate-oxidizing bacterium strain MPOB | - |
Purification (Comment) | Organism |
---|---|
- |
Bacteria |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
971 | - |
- |
Bacteria |
Storage Stability | Organism |
---|---|
4°C, under N2, stable for at least 1 month | Bacteria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate | - |
Bacteria | fumarate + H2O | - |
r | |
fumarate + H2O | - |
Bacteria | L-malate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 60000, SDS-PAGE | Bacteria |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | 55 | - |
Bacteria |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
540 | - |
(S)-malate | - |
Bacteria | |
690 | - |
fumarate | - |
Bacteria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bacteria |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 11.5 | no activity below pH 5.5 and above pH 11.5 | Bacteria |