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Literature summary for 4.2.1.19 extracted from

  • Struhl, K.; Davis, R.W.
    Production of a functional eukaryotic enzyme in Escherichia coli: cloning and expression of the yeast structural gene for imidazoleglycerolphoshate dehydratase (his3) (1977), Proc. Natl. Acad. Sci. USA, 74, 5255-5259.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
3-Amino-1,2,4-triazole Ki: 0.03 mM Saccharomyces cerevisiae
phosphate Ki: 0.008 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate wild-type enzyme Saccharomyces cerevisiae
0.3
-
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate expressed in E. coli Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activity dependent on Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate
-
Saccharomyces cerevisiae 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
wild-type enzyme Saccharomyces cerevisiae
8
-
enzyme expressed in Escherichia coli Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8 wild-type enzyme, less than 50% of maximal activity above and below Saccharomyces cerevisiae
7.2 8.2 enzyme expressed in E. coli, less than 50% of maximal activity above and below Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.008
-
phosphate
-
Saccharomyces cerevisiae
0.03
-
3-Amino-1,2,4-triazole
-
Saccharomyces cerevisiae