Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | - |
Sus scrofa | |
iodoacetamide | 10 mM, 20 min, 20% inhibition | Sus scrofa | |
NEM | 10 mM, 19% inhibition. 5 mM, 13% inhibition | Sus scrofa | |
p-chloromercuribenzoate | 1 mM, complete inhibition. 0.1 mM, 11% inhibition | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
crotonyl-CoA | pH 9.0 | Sus scrofa | |
0.029 | - |
hex-2-enoyl-CoA | pH 9.0 | Sus scrofa | |
0.029 | - |
oct-2-enoyl-CoA | pH 9.0 | Sus scrofa | |
0.029 | - |
dec-2-enoyl-CoA | pH 9.0 | Sus scrofa | |
0.03 | - |
dodec-2-enoyl-CoA | pH 9.0 | Sus scrofa | |
0.03 | - |
hexadec-2-enoyl-CoA | pH 9.0 | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Sus scrofa | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27300 | - |
6 * 27300, SDS-PAGE | Sus scrofa |
155000 | - |
non-denaturing PAGE | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
The classification is ambiguous because the stereochemistry of the reaction product is not exactly determined | - |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1334 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + H2O | best substrate. The classification is ambiguous because the stereochemistry is not exactly determined | Sus scrofa | ? | - |
? | |
dec-2-enoyl-CoA + H2O | 32% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined | Sus scrofa | ? | - |
? | |
dodec-2-enoyl-CoA + H2O | 9.6% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined | Sus scrofa | ? | - |
? | |
hex-2-enoyl-CoA + H2O | 77% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined | Sus scrofa | ? | - |
? | |
hexadec-2-enoyl-CoA + H2O | 2.4% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined | Sus scrofa | ? | - |
? | |
oct-2-enoyl-CoA + H2O | 54% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined | Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 27300, SDS-PAGE | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
crotonase | the classification is ambiguous because the stereochemistry of the reaction product is not exactly determined | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
acetoacetyl-CoA | pH 8.0, due to the binding of the enolate form of acetoacetyl-CoA the Ki-value should be highly pH-dependent | Sus scrofa |