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Literature summary for 4.2.1.164 extracted from

  • Hong L, H.L.; Zhao Z, Z.Z.; Liu H.-W, L.H.
    Characterization of SpnQ from the spinosyn biosynthetic pathway of Saccharopolyspora spinosa: Mechanistic and evolutionary implications for C-3 deoxygenation in deoxysugar biosynthesis (2006), J. Am. Chem. Soc., 128, 14262-14263.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.164

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme contains a [2Fe-2S] binding motif Saccharopolyspora spinosa

Cloned(Commentary)

Cloned (Comment) Organism
expressed in E. coli BL21(DE3) cells Saccharopolyspora spinosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Saccharopolyspora spinosa the enzyme participates in the biosynthesis of forosamine, a key structural component of the spinosyns, which are produced by Saccharopolyspora spinosa dTDP-4-dehydro-2,3,6-trideoxy-alpha-D-hexopyranose + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharopolyspora spinosa Q9ALN8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharopolyspora spinosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ the enzyme participates in the biosynthesis of forosamine, a key structural component of the spinosyns, which are produced by Saccharopolyspora spinosa Saccharopolyspora spinosa dTDP-4-dehydro-2,3,6-trideoxy-alpha-D-hexopyranose + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
-
 ?
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ the enzyme requires ferredoxin/ferredoxin reductase or flavodoxin/flavodoxin reductase. It can act as a transaminase when the electron-transfer path is blocked Saccharopolyspora spinosa dTDP-4-dehydro-2,3,6-trideoxy-alpha-D-hexopyranose + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Saccharopolyspora spinosa

Synonyms

Synonyms Comment Organism
SpnQ
-
 Saccharopolyspora spinosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Saccharopolyspora spinosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharopolyspora spinosa

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Saccharopolyspora spinosa

General Information

General Information Comment Organism
metabolism the enzyme participates in the biosynthesis of forosamine, a key structural component of the spinosyns, which are produced by Saccharopolyspora spinosa Saccharopolyspora spinosa