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Literature summary for 4.2.1.145 extracted from

  • Yin, X.; McPhail, K.L., Kim, K.J.; Zabriskie, T.M.
    Formation of the nonproteinogenic amino acid 2S,3R-capreomycidine by VioD from the viomycin biosynthesis pathway (2004), Chembiochem, 5, 1278-1281.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned into the Escherichia coli expression vector pET28a encoding a N-terminal His6-tag Streptomyces vinaceus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2S,3S)-3-hydroxyarginine Streptomyces vinaceus the enzyme is involved in the biosynthesis of the cyclic pentapeptide antibiotic viomycin (2S,3R)-capreomycidine
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces vinaceus
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-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces vinaceus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,3S)-3-hydroxyarginine the enzyme is involved in the biosynthesis of the cyclic pentapeptide antibiotic viomycin Streptomyces vinaceus (2S,3R)-capreomycidine
-
?
(2S,3S)-3-hydroxyarginine the enzyme catalyzes an intramolecular beta-replacement/elimination reaction. VioD is completely stereospecific for (3S)-hydroxy-L-Arg, as no product formation is observed when (3R)-hydroxy-L-Arg is evaluated as substrate Streptomyces vinaceus (2S,3R)-capreomycidine
-
?

Synonyms

Synonyms Comment Organism
VioD ambiguous Streptomyces vinaceus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Streptomyces vinaceus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Streptomyces vinaceus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate pyridoxal 5'-phosphate dependent enzyme. It is not known if the cofactor is covalently bound to VioD through a lysyl imine, as is common with many PLP-dependent enzymes Streptomyces vinaceus

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of the cyclic pentapeptide antibiotic viomycin Streptomyces vinaceus