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Literature summary for 4.2.1.143 extracted from

  • Silva, J.C.; Townsend, C.A.
    Heterologous expression, isolation, and characterization of versicolorin B synthase from Aspergillus parasiticus. A key enzyme in the aflatoxin B1 biosynthetic pathway (1997), J. Biol. Chem., 272, 804-813.
    View publication on PubMed
Search for more literature for 4.2.1.143

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Aspergillus parasiticus

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3(3-dimethylaminopropyl)carbodiimide 10 mM, 12% residual activity Aspergillus parasiticus
3-(2-hydroxyethyl)-2,3-dihydro-1-benzofuran-2,4-diol 0.05 mM, 30% inhibition. Compound mimics the C-ring and side chain of the substrate Aspergillus parasiticus
diethyl dicarbonate 10 mM, 41% residual activity Aspergillus parasiticus
Hg2+ 1 mM, complete loss of activity Aspergillus parasiticus
additional information not inhibitory: FAD, FMN, riboflavin, and glucose Aspergillus parasiticus
versicolorin B 0.015 mM, 50% inhibition Aspergillus parasiticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0065
-
 versiconal pH 6.5, 37°C Aspergillus parasiticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 x * 78000, SDS-PAGE, recombinant protein, x * 70000, SDS-PAGE of PNGase F treated protein Aspergillus parasiticus
78000
-
 x * 78000, SDS-PAGE, recombinant protein, x * 70000, SDS-PAGE of PNGase F treated protein Aspergillus parasiticus

Organism

Organism UniProt Comment Textmining
Aspergillus parasiticus Q12062
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein sequence contains 3 potential N-glycosylation sites. Treatment with PNGase F leads to a decrease in molecular mass from 78000 kDa for wild-type to 70000 kDa Aspergillus parasiticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2750
-
 pH 6.5, 37°C Aspergillus parasiticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
versiconal
-
 Aspergillus parasiticus versicolorin B + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 78000, SDS-PAGE, recombinant protein, x * 70000, SDS-PAGE of PNGase F treated protein Aspergillus parasiticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 versiconal pH 6.5, 37°C Aspergillus parasiticus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.096
-
 3-(2-hydroxyethyl)-2,3-dihydro-1-benzofuran-2,4-diol pH 6.5, 37°C Aspergillus parasiticus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus parasiticus isoelectric focusing
-
 4.6