Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-(2-hydroxyethyl)-2,3-dihydro-1-benzofuran-2,4-diol | competitive | Aspergillus parasiticus | |
versicolorin B | - |
Aspergillus parasiticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
versiconal hemiacetal | pH 6.5, 37°C | Aspergillus parasiticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
2 * 78000, SDS-PAGE | Aspergillus parasiticus |
140000 | - |
gel filtration | Aspergillus parasiticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus parasiticus | Q12062 | - |
- |
Aspergillus parasiticus ATCC 56775 | Q12062 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus parasiticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1900 | - |
pH 6.5, 37°C | Aspergillus parasiticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme selects from two equilibrating enantiomers of versiconal hemiacetal to cyclize the appropriate antipode to optically pure versicolorin B. By varying the amount of enzyme to a fixed concentration of substrate, the rate of enzymic cyclization can be limited by the intrinsic rate of enantiomerization of the substrate under the conditions of reaction | Aspergillus parasiticus | ? | - |
? | |
additional information | enzyme selects from two equilibrating enantiomers of versiconal hemiacetal to cyclize the appropriate antipode to optically pure versicolorin B. By varying the amount of enzyme to a fixed concentration of substrate, the rate of enzymic cyclization can be limited by the intrinsic rate of enantiomerization of the substrate under the conditions of reaction | Aspergillus parasiticus ATCC 56775 | ? | - |
? | |
versiconal hemiacetal | i.e. (2S,3S)-2,4,6,8-tetrahydroxy-3-(2-hydroxyethyl)anthra[2,3-b]furan-5,10-dione | Aspergillus parasiticus | versicolorin B + H2O | (3aR,12bS)-8,10,12-trihydroxy-1,2,3a,12b-tetrahydroanthra[2,3-b]furo[3,2-d]furan-6,11-dione, | ? | |
versiconal hemiacetal | i.e. (2S,3S)-2,4,6,8-tetrahydroxy-3-(2-hydroxyethyl)anthra[2,3-b]furan-5,10-dione | Aspergillus parasiticus ATCC 56775 | versicolorin B + H2O | (3aR,12bS)-8,10,12-trihydroxy-1,2,3a,12b-tetrahydroanthra[2,3-b]furo[3,2-d]furan-6,11-dione, | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 78000, SDS-PAGE | Aspergillus parasiticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
versiconal hemiacetal | pH 6.5, 37°C | Aspergillus parasiticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | broad | Aspergillus parasiticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | - |
drastic decline in activity below | Aspergillus parasiticus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.076 | - |
3-(2-hydroxyethyl)-2,3-dihydro-1-benzofuran-2,4-diol | pH 6.5, 37°C | Aspergillus parasiticus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus parasiticus | isoelectric focusing | - |
4.7 |