KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
D-galactonate | pH 6.2, 60°C | Picrophilus torridus | |
2.5 | - |
D-gluconate | pH 6.2, 60°C | Picrophilus torridus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, Km: 4.2 mM | Picrophilus torridus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
8 * 44000, SDS-PAGE | Picrophilus torridus |
340000 | - |
gel filtration | Picrophilus torridus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactonate | Picrophilus torridus | - |
2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
D-gluconate | Picrophilus torridus | - |
2-dehydro-3-deoxy-D-gluconate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picrophilus torridus | Q6L1T2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Picrophilus torridus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.1 | - |
substrate: D-gluconate, pH 6.2, 60°C | Picrophilus torridus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactonate | - |
Picrophilus torridus | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
D-galactonate | the ratio of catalytic efficiency for gluconate and galactonate utilization is about 10 to 1 | Picrophilus torridus | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
D-gluconate | - |
Picrophilus torridus | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
D-gluconate | the ratio of catalytic efficiency for gluconate and galactonate utilization is about 10 to 1 | Picrophilus torridus | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
additional information | no activity with xylonate | Picrophilus torridus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homooctamer | 8 * 44000, SDS-PAGE | Picrophilus torridus |
Synonyms | Comment | Organism |
---|---|---|
Pto0485 | - |
Picrophilus torridus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Picrophilus torridus |
70 | - |
- |
Picrophilus torridus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
2 h, stable | Picrophilus torridus |
70 | - |
half-life: 15 min | Picrophilus torridus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Picrophilus torridus |
6.2 | - |
assay at | Picrophilus torridus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.1 | pH 4.5: 50% of maximal activity, pH 8.1: about 50% of maximal activity | Picrophilus torridus |