Literature summary for 4.2.1.136 extracted from

Niehaus, T.D.; Richardson, L.G.; Gidda, S.K.; Elbadawi-Sidhu, M.; Meissen, J.K.; Mullen, R.T.; Fiehn, O.; Hanson, A.D.
Plants utilize a highly conserved system for repair of NADH and NADPH hydrates (2014), Plant Physiol., 165, 52-61.

Search for more literature for 4.2.1.136

Cloned(Commentary)

Cloned (Comment)	Organism
transient expression of GFP-tagged enzyme in Nicotiana tabacum BY-2 cells, expression of N-terminally His-tagged mature dehydratase in Escherichia coli strain BL21 (DE3) RIPL	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of enzyme knockout lines using T-DNA insertion, phenotype, overview	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00069	-	(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate	pH 8.0, 22°C	Arabidopsis thaliana
0.00086	-	(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide	pH 8.0, 22°C	Zea mays
0.00089	-	(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate	pH 8.0, 22°C	Zea mays
0.00115	-	(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide	pH 8.0, 22°C	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Arabidopsis thaliana	9507	-
chloroplast	-	Zea mays	9507	-
cytosol	-	Arabidopsis thaliana	5829	-
cytosol	-	Zea mays	5829	-
mitochondrion	-	Arabidopsis thaliana	5739	-
mitochondrion	-	Zea mays	5739	-
additional information	the NAD(P)HX dehydratase co-localizes with an epimerase, that rapidly interconverts (R)- and (S)-NAD(P)HX	Arabidopsis thaliana	-	-
additional information	the NAD(P)HX dehydratase co-localizes with an epimerase, that rapidly interconverts (R)- and (S)-NAD(P)HX	Zea mays	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Arabidopsis thaliana
Mg2+	required	Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide	Arabidopsis thaliana	-	ADP + phosphate + NADH	-	?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide	Zea mays	-	ADP + phosphate + NADH	-	?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide	Arabidopsis thaliana Col-0	-	ADP + phosphate + NADH	-	?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide	Arabidopsis thaliana Columbia-0	-	ADP + phosphate + NADH	-	?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate	Arabidopsis thaliana	-	ADP + phosphate + NADPH	-	?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate	Zea mays	-	ADP + phosphate + NADPH	-	?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate	Arabidopsis thaliana Col-0	-	ADP + phosphate + NADPH	-	?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate	Arabidopsis thaliana Columbia-0	-	ADP + phosphate + NADPH	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q94AF2	-	-
Arabidopsis thaliana Columbia-0	Q94AF2	-	-
Zea mays	A0A3L6F337	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His-tagged mature dehydratase from Escherichia coli strain BL21 (DE3) RIPL by nickel affinity chromatography	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Arabidopsis thaliana	-
leaf	-	Zea mays	-
seedling	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide	-	Arabidopsis thaliana	ADP + phosphate + NADH	-	?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide	-	Zea mays	ADP + phosphate + NADH	-	?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide	-	Arabidopsis thaliana Col-0	ADP + phosphate + NADH	-	?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide	-	Arabidopsis thaliana Columbia-0	ADP + phosphate + NADH	-	?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate	-	Arabidopsis thaliana	ADP + phosphate + NADPH	-	?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate	-	Zea mays	ADP + phosphate + NADPH	-	?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate	-	Arabidopsis thaliana Col-0	ADP + phosphate + NADPH	-	?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate	-	Arabidopsis thaliana Columbia-0	ADP + phosphate + NADPH	-	?

Synonyms

Synonyms	Comment	Organism
NAD(P)HX dehydratase	-	Arabidopsis thaliana
NAD(P)HX dehydratase	-	Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at	Arabidopsis thaliana
22	-	assay at	Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.26	-	(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide	pH 8.0, 22°C	Arabidopsis thaliana
0.3	-	(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide	pH 8.0, 22°C	Zea mays
0.34	-	(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate	pH 8.0, 22°C	Arabidopsis thaliana
0.38	-	(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate	pH 8.0, 22°C	Zea mays

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Arabidopsis thaliana
8	-	assay at	Zea mays

Cofactor

Cofactor	Comment	Organism	Structure
ADP	dependent on	Arabidopsis thaliana
ADP	dependent on	Zea mays

General Information

General Information	Comment	Organism
malfunction	ablation of the Arabidopsis dehydratase gene raises seedling levels of all NADHX forms by 20 to 40fold, and levels of one NADPHX form by 10 to 30fold	Arabidopsis thaliana
metabolism	plants have a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites	Arabidopsis thaliana
metabolism	plants have a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites	Zea mays
physiological function	NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates [NAD(P)HX], which are not electron donors and inhibit various dehydrogenases. The NAD(P)HX dehydratase is able to reconvert (S)-NAD(P)HX to NAD(P)H in an ATP-dependent manner	Arabidopsis thaliana
physiological function	NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates [NAD(P)HX], which are not electron donors and inhibit various dehydrogenases. The NAD(P)HX dehydratase is able to reconvert (S)-NAD(P)HX to NAD(P)H in an ATP-dependent manner	Zea mays

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
262	-	(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide	pH 8.0, 22°C	Arabidopsis thaliana
358	-	(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide	pH 8.0, 22°C	Zea mays
509	-	(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate	pH 8.0, 22°C	Arabidopsis thaliana
524	-	(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate	pH 8.0, 22°C	Zea mays

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Release 2023.1 (January 2023)