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Literature summary for 4.2.1.134 extracted from

  • Kihara, A.; Sakuraba, H.; Ikeda, M.; Denpoh, A.; Igarashi, Y.
    Membrane topology and essential amino acid residues of Phs1, a 3-hydroxyacyl-CoA dehydratase involved in very long-chain fatty acid elongation (2008), J. Biol. Chem., 283, 11199-11209.
    View publication on PubMed
Search for more literature for 4.2.1.134

Protein Variants

Protein Variants Comment Organism
E116A phytosphingosine accumulates at low levels in the mutant compared with wild type cells Saccharomyces cerevisiae
E156A inactive, the mutant cannot suppress phytosphingosine accumulation, although the protein is expressed normally Saccharomyces cerevisiae
E60A phytosphingosine accumulates at low levels in the mutant compared with wild type cells Saccharomyces cerevisiae
F145A phytosphingosine accumulates at low levels in the mutant compared with wild type cells Saccharomyces cerevisiae
G152A mutant with reduced activity, the mutant cannot suppress phytosphingosine accumulation, although the protein is expressed normally Saccharomyces cerevisiae
L12A phytosphingosine accumulates at low levels in the mutant compared with wild type cells Saccharomyces cerevisiae
N16A phytosphingosine accumulates at low levels in the mutant compared with wild type cells Saccharomyces cerevisiae
P150A phytosphingosine accumulates at low levels in the mutant compared with wild type cells Saccharomyces cerevisiae
P188A phytosphingosine accumulates at low levels in the mutant compared with wild type cells Saccharomyces cerevisiae
Q200A phytosphingosine accumulates at low levels in the mutant compared with wild type cells Saccharomyces cerevisiae
Q54A phytosphingosine accumulates at low levels in the mutant compared with wild type cells Saccharomyces cerevisiae
Q79A inactive, the mutant cannot suppress phytosphingosine accumulation, although the protein is expressed normally Saccharomyces cerevisiae
R119A phytosphingosine accumulates at low levels in the mutant compared with wild type cells Saccharomyces cerevisiae
R141A mutant with reduced activity, the mutant cannot suppress phytosphingosine accumulation, although the protein is expressed normally Saccharomyces cerevisiae
R201A phytosphingosine accumulates at low levels in the mutant compared with wild type cells Saccharomyces cerevisiae
R83A mutant with reduced activity, the mutant cannot suppress phytosphingosine accumulation, although the protein is expressed normally Saccharomyces cerevisiae
W124A phytosphingosine accumulates at low levels in the mutant compared with wild type cells Saccharomyces cerevisiae
W23A phytosphingosine accumulates at low levels in the mutant compared with wild type cells Saccharomyces cerevisiae
Y142A phytosphingosine accumulates at low levels in the mutant compared with wild type cells Saccharomyces cerevisiae
Y149A inactive, the mutant cannot suppress phytosphingosine accumulation, although the protein is expressed normally Saccharomyces cerevisiae
Y15A phytosphingosine accumulates at low levels in the mutant compared with wild type cells Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane a membranespanning protein that traverses the membrane six times and has an N-terminus and C-terminus facing the cytosol Saccharomyces cerevisiae 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxyacyl-CoA Saccharomyces cerevisiae
-
 2,3-trans-enoyl-CoA + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxyacyl-CoA
-
 Saccharomyces cerevisiae 2,3-trans-enoyl-CoA + H2O
-
 ?
3-hydroxypalmitoyl-CoA
-
 Saccharomyces cerevisiae 2,3-trans-hexadecenoyl-CoA + H2O
-
 ?

Synonyms

Synonyms Comment Organism
3-hydroxyacyl-CoA dehydratase
-
 Saccharomyces cerevisiae
PHS1
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction reduced enzyme levels result in significant impairment of the conversion of ceramide to inositol phosphorylceramide, cause an accumulation of ceramide and a reduction in complex sphingolipids Saccharomyces cerevisiae
metabolism the enzyme catalyzes the third reaction of the four-step cycle in the elongation of very long-chain fatty acids Saccharomyces cerevisiae