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Literature summary for 4.2.1.130 extracted from

  • Kwon, K.; Choi, D.; Hyun, J.K.; Jung, H.S.; Baek, K.; Park, C.
    Novel glyoxalases from Arabidopsis thaliana (2013), FEBS J., 280, 3328-3339.
    View publication on PubMed
Search for more literature for 4.2.1.130

Cloned(Commentary)

Cloned (Comment) Organism
gene DJ1A, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3) Arabidopsis thaliana
gene DJ1B, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3) Arabidopsis thaliana
gene DJ1C, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3) Arabidopsis thaliana
gene DJ1E, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3) Arabidopsis thaliana
gene DJ1F, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of HIs-tagged enzyme in Escherichia coli strain BL21 (DE3) Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41400
-
-
 Arabidopsis thaliana
41600
-
-
 Arabidopsis thaliana
41900
-
-
 Arabidopsis thaliana
42700
-
-
 Arabidopsis thaliana
43100
-
-
 Arabidopsis thaliana
80000
-
 gel filtration, recombinant enzyme Arabidopsis thaliana
84000
-
 gel filtration, recombinant enzyme Arabidopsis thaliana
126000
-
 gel filtration, recombinant enzyme Arabidopsis thaliana
132000
-
 gel filtration, recombinant enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxopropanal + H2O Arabidopsis thaliana
-
 (R)-lactate
-
 ?
2-oxopropanal + H2O Arabidopsis thaliana Col-0
-
 (R)-lactate
-
 ?
glyoxal + H2O Arabidopsis thaliana
-
 acetate
-
 ?
glyoxal + H2O Arabidopsis thaliana Col-0
-
 acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8VY09
-
-
Arabidopsis thaliana Q9FPF0
-
-
Arabidopsis thaliana Q9M1G8
-
-
Arabidopsis thaliana Q9MAH3
-
-
Arabidopsis thaliana Q9ZV19
-
-
Arabidopsis thaliana Col-0 Q8VY09
-
-
Arabidopsis thaliana Col-0 Q9FPF0
-
-
Arabidopsis thaliana Col-0 Q9M1G8
-
-
Arabidopsis thaliana Col-0 Q9MAH3
-
-
Arabidopsis thaliana Col-0 Q9ZV19
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the enzyme DJ-1C shows very low enzyme activity Arabidopsis thaliana
additional information
-
 the enzyme DJ-1E shows very low activity with substrate glyoxal Arabidopsis thaliana
0.0055
-
 substrate methylglyoxal, pH 6.8, 37°C Arabidopsis thaliana
0.0062
-
 substrate methylglyoxal, pH 6.8, 37°C Arabidopsis thaliana
0.013
-
 substrate methylglyoxal, pH 6.8, 37°C Arabidopsis thaliana
0.015
-
 substrate methylglyoxal, pH 6.8, 37°C Arabidopsis thaliana
0.25
-
 substrate glyoxal, pH 6.8, 37°C Arabidopsis thaliana
0.31
-
 substrate glyoxal, pH 6.8, 37°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxopropanal + H2O
-
 Arabidopsis thaliana (R)-lactate
-
 ?
2-oxopropanal + H2O
-
 Arabidopsis thaliana Col-0 (R)-lactate
-
 ?
glyoxal + H2O
-
 Arabidopsis thaliana acetate
-
 ?
glyoxal + H2O
-
 Arabidopsis thaliana Col-0 acetate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 41400, about, sequence calculation Arabidopsis thaliana
dimer 2 * 41900, about, sequence calculation Arabidopsis thaliana
More three-dimensional structure comparisons of DJ-1 proteins from Arabidopsis taliana, molecular modelling, overview. Secondary structure content estimated by circular dichroism Arabidopsis thaliana
trimer 3 * 41600, about, sequence calculation Arabidopsis thaliana
trimer 3 * 42700, about, sequence calculation Arabidopsis thaliana
trimer 3 * 43100, about, sequence calculation Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
DJ-1a
-
 Arabidopsis thaliana
DJ-1b
-
 Arabidopsis thaliana
DJ-1c
-
 Arabidopsis thaliana
DJ-1e
-
 Arabidopsis thaliana
DJ-1f
-
 Arabidopsis thaliana
protein DJ-1 homolog A UniProt Arabidopsis thaliana
protein DJ-1 homolog B UniProt Arabidopsis thaliana
protein DJ-1 homolog C UniProt Arabidopsis thaliana
protein DJ-1 homolog E UniProt Arabidopsis thaliana
protein DJ-1 homolog F UniProt Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
evolution the enzyme belongs to the DJ-1/PfpI superfamily. Unlike the homologues from other species, all the Arabidopsis thaliana genes have two tandem domains, which may have been created by gene duplication. The six AtDJ-1 proteins (a-f) are characterized Arabidopsis thaliana