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Literature summary for 4.2.1.121 extracted from

  • Itoh, A.; Howe, G.A.
    Molecular cloning of a divinyl ether synthase. Identification as a CYP74 cytochrome P-450 (2001), J. Biol. Chem., 276, 3620-3627.
    View publication on PubMed
Search for more literature for 4.2.1.121

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate pH 7.0, 25°C Solanum lycopersicum
0.067
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate pH 7.0, 25°C Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55254
-
 x * 55254, calculated from sequence Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum Q9FPM6 LeDES is a single copy gene located on the distal half of the long arm of chromosome one; cv. Castlemart
-

Purification (Commentary)

Purification (Comment) Organism
-
 Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
root LeDES transcripts are most abundant in roots. A low level of LeDES mRNA is observed in stem tissue, but no accumulation is detected in flower buds, petioles, cotyledons, or leaves. Extracts from roots of young plants, but not extracts from stem or leaf tissue, catalyze efficient formation of colneleate from the hydroperoxide precursor Solanum lycopersicum
-
stem LeDES transcripts are most abundant in roots. A low level of LeDES mRNA is observed in stem tissue, but no accumulation is detected in flower buds, petioles, cotyledons, or leaves. Extracts from roots of young plants, but not extracts from stem or leaf tissue, catalyze efficient formation of colneleate from the hydroperoxide precursor Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate poorly active against the the corresponding 13-hydroperoxide Solanum lycopersicum (8E)-9-[(1E,3Z)-nona-1,3-dien-1-yloxy]non-8-enoic acid + H2O i.e. colneleate, characterization of the product ?
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate poorly active against the the corresponding 13-hydroperoxide Solanum lycopersicum (8E)-9-[(1E,3Z,6Z)-nona-1,3,6-trien-1-yloxy]non-8-enoic acid + H2O i.e. colnelenate ?

Subunits

Subunits Comment Organism
? x * 55254, calculated from sequence Solanum lycopersicum

Synonyms

Synonyms Comment Organism
CYP74 cytochrome P-450
-
 Solanum lycopersicum
CYP74D1
-
 Solanum lycopersicum
LeDES
-
 Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
500
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate pH 7.0, 25°C Solanum lycopersicum
890
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate pH 7.0, 25°C Solanum lycopersicum

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450 the enzyme has spectral properties of cytochrome P-450 Solanum lycopersicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10420
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate pH 7.0, 25°C Solanum lycopersicum
13280
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate pH 7.0, 25°C Solanum lycopersicum