Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant C-terminally His6-tagged MFE2s in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
R251A | site-directed mutagenesis, the mutant shows decreased labeling efficiency in photoaffinity labeling of substrate binding sites with photophores compared to the wild-type enzyme, overview | Rattus norvegicus |
W249A | site-directed mutagenesis, the mutant shows decreased labeling efficiency in photoaffinity labeling of substrate binding sites with photophores compared to the wild-type enzyme, overview | Rattus norvegicus |
W249G | site-directed mutagenesis, the mutant shows decreased labeling efficiency in photoaffinity labeling of substrate binding sites with photophores compared to the wild-type enzyme, overview | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisomal matrix | MFE2 possesses a typical peroxisomal matrix protein targeting signal, COOH-terminal Ala-Lys-Leu | Rattus norvegicus | 5782 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, SDS-PAGE | Rattus norvegicus |
155000 | - |
recombinant enzyme, sucrose density gradient centrifugation | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus norvegicus Wistar | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | identification of substrate binding sites, residues Trp249 to Arg251, using a photoreactive palmitic acid analogue bearing a diazirine moiety as a photophore in photoaffinity labeling of purified rat liver peroxisomes, ligand preparation, overview. The labeling efficiency competitively decreases in the presence of palmitoyl-CoA | Rattus norvegicus | ? | - |
? | |
additional information | identification of substrate binding sites, residues Trp249 to Arg251, using a photoreactive palmitic acid analogue bearing a diazirine moiety as a photophore in photoaffinity labeling of purified rat liver peroxisomes, ligand preparation, overview. The labeling efficiency competitively decreases in the presence of palmitoyl-CoA | Rattus norvegicus Wistar | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Rattus norvegicus |
More | MFE2 consists of a (3R)-hydroxyacyl-CoA dehydrogenase, HD, domain, a (2E)-enoyl-CoA hydratase 2, H2, domain, and a sterol carrier protein 2-like domain | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
MFE2 | - |
Rattus norvegicus |
multifunctional enzyme type 2 | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the peroxisomal beta-oxidation of fatty acids and their derivatives | Rattus norvegicus |
physiological function | MFE2 consists of a (3R)-hydroxyacyl-CoA dehydrogenase, HD, domain, a (2E)-enoyl-CoA hydratase 2, H2, domain, and a sterol carrier protein 2-like domain, and is known to catalyze the second and third steps of the peroxisomal beta-oxidation of fatty acids and their derivatives | Rattus norvegicus |