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Literature summary for 4.2.1.119 extracted from

  • Koski, M.K.; Haapalainen, A.M.; Hiltunen, J.K.; Glumoff, T.
    Crystallization and preliminary crystallographic data of 2-enoyl-CoA hydratase 2 domain of Candida tropicalis peroxisomal multifunctional enzyme type 2 (2003), Acta Crystallogr. Sect. D, 59, 1302-1305.
    View publication on PubMed
Search for more literature for 4.2.1.119

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Candida tropicalis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour-diffusion method. Crystals of native and SeMet CtMfe2p(dha+bdelta) Candida tropicalis

Protein Variants

Protein Variants Comment Organism
additional information CtMfe2p(dha+bdelta) labelled with selenomethionine (SeMet), the plasmid pET3a::CtMfe2p(dha+bdelta) is transformed to the methionine-auxotrophic Escherichia coli strain B834(DE3). The incorporation of SeMet into the structure does not affect the hydratase 2 activity Candida tropicalis

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 both recombinant CtMfe2p(dha+bdelta) and its SeMet analogue, SDS-PAGE Candida tropicalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Candida tropicalis in yeast, the second and the third reaction of the fatty-acid beta-oxidation spiral are catalysed by peroxisomal multifunctional enzyme type 2 (Mfe2p/Fox2p). This protein has two (3R)-hydroxyacyl-CoA dehydrogenase domains and a C-terminal 2-enoyl-CoA hydratase 2 domain ?
-
 ?

Organism

Organism UniProt Comment Textmining
Candida tropicalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Candida tropicalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-2-decenoyl-CoA + H2O activity measurements are based on the formation of the magnesium complex of 3-ketoacyl-CoA from (2E)-2-decenoyl-CoA Candida tropicalis (3R)-3-hydroxydecanoyl-CoA
-
 ?
additional information in yeast, the second and the third reaction of the fatty-acid beta-oxidation spiral are catalysed by peroxisomal multifunctional enzyme type 2 (Mfe2p/Fox2p). This protein has two (3R)-hydroxyacyl-CoA dehydrogenase domains and a C-terminal 2-enoyl-CoA hydratase 2 domain Candida tropicalis ?
-
 ?
additional information MFE-2 is a multifunctional enzyme with 2-enoyl-CoA hydratase 2 activity and 2/(3R)-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.36) activity Candida tropicalis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 63000, both recombinant CtMfe2p(dha+bdelta) and its SeMet analogue, SDS-PAGE Candida tropicalis

Synonyms

Synonyms Comment Organism
2-enoyl-CoA hydratase 2
-
 Candida tropicalis
MFE-2 multifunctional enzyme Candida tropicalis
MFE-2 multifunctional enzyme with 2-enoyl-CoA hydratase 2 activity and 2/(3R)-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.36) activity Candida tropicalis
Mfe2p [CtMfe2p(dha+bdelta)] 2-enoyl-CoA hydratase 2 domain of Candida tropicalis Candida tropicalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
388
-
 (2E)-2-decenoyl-CoA recombinant enzyme CtMfe2p(dha+bdelta) Candida tropicalis