Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | enzyme different from peroxisomal hydratase 2 though their kinetic properties are similar. They differ in immunological data, subunit size, and chromatographic behaviour, the peroxisomal isoform is not recognized by the antibody to its microsomal counterpart | Rattus norvegicus | - |
- |
peroxisome | enzyme different from microsomal hydratase 2 though their kinetic properties are similar. They differ in immunological data, subunit size, and chromatographic behaviour, the peroxisomal isoform is not recognized by the antibody to its microsomal counterpart | Rattus norvegicus | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31500 | - |
2 * 31500, microsomal isoform, SDS-PAGE | Rattus norvegicus |
33500 | - |
2 * 33500, peroxisomal isoform, SDS-PAGE | Rattus norvegicus |
60000 | - |
microsomal isoform, gel filtration | Rattus norvegicus |
62000 | - |
peroxisomal isoform, gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
0.2% yield,the microsomal isoform elutes at 0.4-0.7 MKCl, at low salt concentrations, whereas the peroxisomal isoform elutes at high salt concentrations | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
37.5 | - |
purified enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxydecanoyl-CoA | - |
Rattus norvegicus | trans-2-decenoyl-CoA + H2O | - |
r | |
additional information | the (S)-3-hydroxy-CoA is not dehydrated | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 31500, microsomal isoform, SDS-PAGE | Rattus norvegicus |
homodimer | 2 * 33500, peroxisomal isoform, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Rattus norvegicus |