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Literature summary for 4.2.1.119 extracted from

  • Fukui, T.; Shiomi, N.; Doi, Y.
    Expression and characterization of (R)-specific enoyl coenzyme A hydratase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae (1998), J. Bacteriol., 180, 667-673.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.119

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Aeromonas caviae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 crotonyl-CoA pH 8.0, 30°C Aeromonas caviae
0.034
-
 hex-2-enoyl-CoA pH 8.0, 30°C Aeromonas caviae
0.036
-
 pent-2-enoyl-CoA pH 8.0, 30°C Aeromonas caviae
0.05
-
 oct-2-enoyl-CoA pH 8.0, 30°C Aeromonas caviae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13954
-
 2 * 13954, calculated from sequence Aeromonas caviae
30000
-
 gel filtration Aeromonas caviae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aeromonas caviae channelling pathway for supplying (R)-3-hydroxyacyl-CoA monomer units from fatty acid beta-oxidation to poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aeromonas caviae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonyl-CoA + H2O
-
 Aeromonas caviae (R)-3-hydroxybutanoyl-CoA
-
 ?
hex-2-enoyl-CoA + H2O
-
 Aeromonas caviae (R)-3-hydroxyhexanoyl-CoA
-
 ?
additional information channelling pathway for supplying (R)-3-hydroxyacyl-CoA monomer units from fatty acid beta-oxidation to poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis Aeromonas caviae ?
-
 ?
oct-2-enoyl-CoA + H2O
-
 Aeromonas caviae (R)-3-hydroxyoctanoyl-CoA
-
 ?
pent-2-enoyl-CoA + H2O
-
 Aeromonas caviae (R)-3-hydroxypentanoyl-CoA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 13954, calculated from sequence Aeromonas caviae

Synonyms

Synonyms Comment Organism
(R)-specific enoyl coenzyme A hydratase
-
 Aeromonas caviae
phaJ
-
 Aeromonas caviae
PhaJAc
-
 Aeromonas caviae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Aeromonas caviae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Aeromonas caviae