Crystallization (Comment) | Organism |
---|---|
striking differences in overall structure, altered oligomeric structure, length variation, orientation of the capping domain, active site structure, differences in substrate binding, conformation and position of the 20s loop structure, evolution of new protein functions by promiscuous binding and reactions with new substrates due to structural flexibility | Amycolatopsis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate affinity is as much as 40fold higher for Escherichia coli OSBS than for OSBS of Amycolatopsis, also stated by analysis on phylogeny, structure, sequence conservation | Amycolatopsis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate | Amycolatopsis sp. | menaquinone pathway | 4-(2-carboxyphenyl)-4-oxobutanoic acid + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis sp. | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specificity maintained with little conservation of ligand-contacting residues despite of extensive structural variation in orthologous proteins | Amycolatopsis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate | menaquinone pathway | Amycolatopsis sp. | 4-(2-carboxyphenyl)-4-oxobutanoic acid + H2O | - |
? | |
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate | menaquinone pathway, presence in species lacking the menaquinone pathway suggests additional roles in amino acid racemization | Amycolatopsis sp. | 4-(2-carboxyphenyl)-4-oxobutanoic acid + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
o-succinylbenzoate synthase | - |
Amycolatopsis sp. |
OSBS | - |
Amycolatopsis sp. |