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Literature summary for 4.2.1.112 extracted from

  • Seiffert, G.B.; Ullmann, G.M.; Messerschmidt, A.; Schink, B.; Kroneck, P.M.H.; Einsle, O.
    Structure of the non-redox-active tungsten/[4Fe:4S] enzyme acetylene hydratase (2007), Proc. Natl. Acad. Sci. USA, 104, 3073-3077.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.112

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme, the mother liquor contains plus 15% (v/v) 2-methyl-2,5-pentanediol, X-ray diffraction structure determination and analysis at 1.26-1.95 A resolution, modeling Syntrophotalea acetylenica

Metals/Ions

Metals/Ions Comment Organism Structure
Iron non-redox-active tungsten/[4Fe-4S] enzyme, a cubane-type [4Fe:4S] cluster, structure analysis, overview Syntrophotalea acetylenica
Tungsten a non-redox-active tungsten/[4Fe-4S] enzyme, contains two molybdopterin guanine dinucleotide cofactors, MGD, designated P and Q, structure analysis, bis-molybdopterin guanine dinucleotide-ligated tungsten atom, overview, the tungsten center binds a water molecule that is activated by an adjacent aspartate residue, enabling to attack acetylene bound in a distinct, hydrophobic pocket, this mechanism requires a strong shift of pKa of the aspartate, caused by nearby low-potential [4Fe:4S] cluster, overview Syntrophotalea acetylenica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylene + H2O Syntrophotalea acetylenica
-
 acetaldehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Syntrophotalea acetylenica
-
-
-

Reaction

Reaction Comment Organism Reaction ID
acetaldehyde = acetylene + H2O active site channel structure and detailed catalytic mechanism Syntrophotalea acetylenica
a

Source Tissue

Source Tissue Comment Organism Textmining
additional information strictly anaerobic growth conditions Syntrophotalea acetylenica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylene + H2O
-
 Syntrophotalea acetylenica acetaldehyde
-
 ?
acetylene + H2O a non-redox reaction Syntrophotalea acetylenica acetaldehyde
-
 ?

Subunits

Subunits Comment Organism
More four domain structure with active site access pathway, active site architecture, overview Syntrophotalea acetylenica

Synonyms

Synonyms Comment Organism
More the enzyme structurally belongs to the dimethyl sulfoxide reductase family of enzymes Syntrophotalea acetylenica