Cloned (Comment) | Organism |
---|---|
gene tetur28g02430, sequence comparisons and phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli BL21(DE3) pLys, the Tetranychus urticae cyanase remains functionally active after horizontal gene transfer | Tetranychus urticae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sodium chloride | - |
Tetranychus urticae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Tetranychus urticae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tetranychus urticae | T1KZQ3 | gene tetur28g02430 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli BL21(DE3) pLys by nickel affinity chromatography and dialysis | Tetranychus urticae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Tetranychus urticae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Tetranychus urticae |
General Information | Comment | Organism |
---|---|---|
evolution | an ancient gene transfer occurred before the diversification within the Tetranychidae family | Tetranychus urticae |