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Literature summary for 4.2.1.104 extracted from

  • Qian, D.; Jiang, L.; Lu, L.; Wei, C.; Li, Y.
    Biochemical and structural properties of cyanases from Arabidopsis thaliana and Oryza sativa (2011), PLoS ONE, 6, e18300.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.104

Cloned(Commentary)

Cloned (Comment) Organism
gene Atcyn, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21 (DE3) as His-tagged soluble protein Arabidopsis thaliana
gene Oscyn, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21 (DE3) as His-tagged soluble protein Oryza sativa

Protein Variants

Protein Variants Comment Organism
E94L site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme. The mutant can form trimers or a mixture of polymers but not decamers Arabidopsis thaliana
additional information construction of CYN knockout plants Arabidopsis thaliana
S117A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme. The mutant can form trimers or a mixture of polymers but not decamers Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21900
-
 10 * 21900, about, sequence calculation, homology modelling of monomers, coimmunoprecipitation, and gel filtration Arabidopsis thaliana
22000
-
 10 * 22000, about, sequence calculation, homology modelling of monomers, coimmunoprecipitation, and gel filtration Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyanate + HCO3- + 2 H+ Arabidopsis thaliana
-
 NH3 + 2 CO2
-
 ?
cyanate + HCO3- + 2 H+ Oryza sativa
-
 NH3 + 2 CO2
-
 ?
cyanate + HCO3- + 2 H+ Arabidopsis thaliana Col 0
-
 NH3 + 2 CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O22683
-
-
Arabidopsis thaliana Col 0 O22683
-
-
Oryza sativa Q9FWK4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged soluble CYN from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Arabidopsis thaliana
recombinant His-tagged soluble CYN from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
flower the transcription level of AtCYN is higher in the flower than in other organs of Arabidopsis thaliana Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.038
-
 purified recombinant mutant E94L, pH 7.7, 27°C Arabidopsis thaliana
0.04
-
 purified recombinant mutant S117A, pH 7.7, 27°C Arabidopsis thaliana
0.303
-
 purified recombinant enzyme, pH 4.8, 27°C Oryza sativa
0.633
-
 purified recombinant enzyme, pH 5.7, 27°C Oryza sativa
2.286
-
 purified recombinant wild-type enzyme, pH 7.7, 27°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanate + HCO3- + 2 H+
-
 Arabidopsis thaliana NH3 + 2 CO2
-
 ?
cyanate + HCO3- + 2 H+
-
 Oryza sativa NH3 + 2 CO2
-
 ?
cyanate + HCO3- + 2 H+
-
 Arabidopsis thaliana Col 0 NH3 + 2 CO2
-
 ?

Subunits

Subunits Comment Organism
homodecamer 10 * 21900, about, sequence calculation, homology modelling of monomers, coimmunoprecipitation, and gel filtration Arabidopsis thaliana
homodecamer 10 * 22000, about, sequence calculation, homology modelling of monomers, coimmunoprecipitation, and gel filtration Oryza sativa

Synonyms

Synonyms Comment Organism
AtCYN
-
 Arabidopsis thaliana
cyanate hydrolase
-
 Arabidopsis thaliana
cyanate hydrolase
-
 Oryza sativa
cyanate lyase
-
 Arabidopsis thaliana
cyanate lyase
-
 Oryza sativa
OsCYN
-
 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
 assay at Arabidopsis thaliana
27
-
 assay at Oryza sativa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
19 34 the enzyme activity increases concomitantly with increasing temperature, the activity is 2fold higher at 34°C compared to 19°C Arabidopsis thaliana
19 34 the enzyme activity increases concomitantly with increasing temperature, the activity is 2fold higher at 34°C compared to 19°C Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7
-
-
 Oryza sativa
7.7
-
-
 Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
4.8 8.8 active within that range Oryza sativa
6.7 8.8 over 75% of maximal activity at pH 6.7-8.8, no activity at pH 5.7 and pH 4.8 Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana AtCYN transcription is not significantly affected by KCNO treatment, but is induced by salt stress up

General Information

General Information Comment Organism
malfunction cyanate treatment inhibits germination and early seedling growth of Cyn knockout mutant plants, while wild-type plants show resistance to cyanate stress Arabidopsis thaliana
physiological function one role of cyanases in plants is detoxification, plants containing CYN exhibit resistance to KCNO stress Oryza sativa
physiological function one role of cyanases in plants is detoxification, plants containing CYN exhibit resistance to KCNO stress. The conserved residues Ser117 and Glu94 are not only catalytic residues in AtCYN but also contribute to the stability of AtCYN homodecamers. Transcriptional regulation and expression pattern of AtCYN, overview Arabidopsis thaliana