Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 1 mM, 99% inhibition | Pseudomonas putida | |
ammonium persulfate | 1 mM, 23% inhibition | Pseudomonas putida | |
CdCl2 | 1 mM, 87% inhibition | Pseudomonas putida | |
CoCl2 | 1 mM, 98% inhibition | Pseudomonas putida | |
CuSO4 | 1 mM, 98% inhibition | Pseudomonas putida | |
Diethyldithiocarbamic acid sodium salt | 1 mM, 20% inhibition | Pseudomonas putida | |
FeSO4 | 1 mM, 50% inhibition | Pseudomonas putida | |
H2O2 | 1 mM, 49% inhibition | Pseudomonas putida | |
HgCl2 | 1 mM, 99% inhibition | Pseudomonas putida | |
hydroxylamine | 1 mM, 10% inhibition | Pseudomonas putida | |
iodoacetate | 1 mM, 97% inhibition | Pseudomonas putida | |
KCN | 1 mM, 26% inhibition | Pseudomonas putida | |
NEM | 1 mM,51% inhibition | Pseudomonas putida | |
NiSO4 | 1 mM, 98% inhibition | Pseudomonas putida | |
PCMB | 1 mM, 50% inhibition | Pseudomonas putida | |
phenylmethanesulfonyl fluoride | 1 mM, 57% inhibition | Pseudomonas putida | |
SnCl2 | 1 mM, 16% inhibition | Pseudomonas putida | |
ZnCl2 | 1 mM, 34% inhibition | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.2 | - |
cyclohexyl isocyanide | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
2 * 59000, SDS-PAGE | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexyl isocyanide + H2O | Pseudomonas putida | enzyme is involved in nitrogen-carbon triple bond cleavage | N-cyclohexylformamide | - |
? | |
cyclohexyl isocyanide + H2O | Pseudomonas putida N19-2 | enzyme is involved in nitrogen-carbon triple bond cleavage | N-cyclohexylformamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
N19-2 | - |
Pseudomonas putida N19-2 | - |
N19-2 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyl isocyanide | 224% of the activity with cyclohexyl isocyanide | Pseudomonas putida | N-phenylformamide | - |
? | |
benzyl isocyanide | 224% of the activity with cyclohexyl isocyanide | Pseudomonas putida N19-2 | N-phenylformamide | - |
? | |
cyclohexyl isocyanide + H2O | - |
Pseudomonas putida | N-cyclohexylformamide | - |
? | |
cyclohexyl isocyanide + H2O | enzyme is involved in nitrogen-carbon triple bond cleavage | Pseudomonas putida | N-cyclohexylformamide | - |
? | |
cyclohexyl isocyanide + H2O | - |
Pseudomonas putida N19-2 | N-cyclohexylformamide | - |
? | |
cyclohexyl isocyanide + H2O | enzyme is involved in nitrogen-carbon triple bond cleavage | Pseudomonas putida N19-2 | N-cyclohexylformamide | - |
? | |
isocyanomethyl phosphonic acid diethyl ether + H2O | 3% of the activity with cyclohexyl isocyanide | Pseudomonas putida | formylaminomethyl-phosphonic acid diethyl ester | - |
? | |
isocyanomethyl phosphonic acid diethyl ether + H2O | 3% of the activity with cyclohexyl isocyanide | Pseudomonas putida N19-2 | formylaminomethyl-phosphonic acid diethyl ester | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 59000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Pseudomonas putida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | 20°C: about 55% of maximal activity, 45°C: about 55% of maximal activity | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
30 min, 10% v/v glycerol, 3% loss of activity | Pseudomonas putida |
20 | - |
30 min, 10% v/v glycerol, 11% loss of activity | Pseudomonas putida |
30 | - |
30 min, 10% v/v glycerol, 16% loss of activity | Pseudomonas putida |
35 | - |
30 min, 10% v/v glycerol, 22% loss of activity | Pseudomonas putida |
40 | - |
30 min, 10% v/v glycerol, 31% loss of activity | Pseudomonas putida |
45 | - |
30 min, 10% v/v glycerol, 47% loss of activity | Pseudomonas putida |
50 | - |
30 min, 10% v/v glycerol, 67% loss of activity | Pseudomonas putida |
55 | - |
30 min, 10% v/v glycerol, 81% loss of activity | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | pH 5.5: about 45% of maximal activity, potassium phosphate buffer, pH 9.0: about 30% of maximal activity, NH4OH/NH4Cl buffer | Pseudomonas putida |