Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)pLysS cells | Syntrophus aciditrophicus |
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)pLysS cells | Geobacter metallireducens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
cyclohexa-1,5-diene-1-carbonyl-CoA | pH 7.5, 30°C | Syntrophus aciditrophicus | |
0.05 | - |
6-hydroxycyclohex-1-enecarbonyl-CoA | - |
Geobacter metallireducens | |
0.085 | - |
cyclohexa-1,5-diene-1-carbonyl-CoA | pH 7.5, 30°C | Geobacter metallireducens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29900 | - |
4 * 30000, SDS-PAGE of purified His-tag labeled protein, 4 * 29900, calculated mass of the His-tag labeled protein, native mass by gel filtration | Syntrophus aciditrophicus |
30000 | - |
2 * 30000, SDS-PAGE of purified His-tag labeled protein, 2 * 30400, calculated mass of the His-tag labeled protein, native mass by Ferguson blot analysis | Geobacter metallireducens |
30000 | - |
3 * 30000, SDS-PAGE of purified His-tag labeled protein, 3 * 30400, calculated mass of the His-tag labeled protein, native mass by gel filtration | Geobacter metallireducens |
30000 | - |
4 * 30000, SDS-PAGE of purified His-tag labeled protein, 4 * 29900, calculated mass of the His-tag labeled protein, native mass by gel filtration | Syntrophus aciditrophicus |
30400 | - |
2 * 30000, SDS-PAGE of purified His-tag labeled protein, 2 * 30400, calculated mass of the His-tag labeled protein, native mass by Ferguson blot analysis | Geobacter metallireducens |
30400 | - |
3 * 30000, SDS-PAGE of purified His-tag labeled protein, 3 * 30400, calculated mass of the His-tag labeled protein, native mass by gel filtration | Geobacter metallireducens |
70000 | - |
Ferguson blot analysis | Geobacter metallireducens |
92000 | - |
gel filtration | Geobacter metallireducens |
115000 | - |
gel filtration | Syntrophus aciditrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O | Syntrophus aciditrophicus | involved in the benzoyl-CoA pathway | 6-hydroxycyclohex-1-enecarbonyl-CoA | - |
r | |
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O | Desulfococcus multivorans | involved in the benzoyl-CoA pathway | 6-hydroxycyclohex-1-enecarbonyl-CoA | - |
r | |
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O | Geobacter metallireducens | involved in the benzoyl-CoA pathway | 6-hydroxycyclohex-1-enecarbonyl-CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfococcus multivorans | - |
strain DSMZ 2059, grown with benzoate as substrate | - |
Geobacter metallireducens | - |
strain DSMZ 7210, grown with benzoate as substrate | - |
Syntrophus aciditrophicus | - |
grown with benzoate as substrate | - |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Syntrophus aciditrophicus |
recombinant protein using His-tag | Geobacter metallireducens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.8 | - |
cell extract, pH 7.5, 30°C | Syntrophus aciditrophicus |
2 | - |
cell extract, pH 7.5, 30°C | Desulfococcus multivorans |
2.5 | - |
cell extract, pH 7.5, 30°C | Geobacter metallireducens |
350 | - |
purified enzyme, hydration reaction, pH 7.5, 30°C | Syntrophus aciditrophicus |
550 | - |
purified enzyme, hydration reaction, pH 7.5, 30°C | Geobacter metallireducens |
730 | - |
purified enzyme, dehydration reaction, pH 7.5, 30°C | Geobacter metallireducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O | - |
Syntrophus aciditrophicus | 6-hydroxycyclohex-1-enecarbonyl-CoA | - |
r | |
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O | - |
Desulfococcus multivorans | 6-hydroxycyclohex-1-enecarbonyl-CoA | - |
r | |
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O | - |
Geobacter metallireducens | 6-hydroxycyclohex-1-enecarbonyl-CoA | - |
r | |
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O | involved in the benzoyl-CoA pathway | Syntrophus aciditrophicus | 6-hydroxycyclohex-1-enecarbonyl-CoA | - |
r | |
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O | involved in the benzoyl-CoA pathway | Desulfococcus multivorans | 6-hydroxycyclohex-1-enecarbonyl-CoA | - |
r | |
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O | involved in the benzoyl-CoA pathway | Geobacter metallireducens | 6-hydroxycyclohex-1-enecarbonyl-CoA | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, SDS-PAGE of purified His-tag labeled protein, 2 * 30400, calculated mass of the His-tag labeled protein, native mass by Ferguson blot analysis | Geobacter metallireducens |
tetramer | 4 * 30000, SDS-PAGE of purified His-tag labeled protein, 4 * 29900, calculated mass of the His-tag labeled protein, native mass by gel filtration | Syntrophus aciditrophicus |
trimer | 3 * 30000, SDS-PAGE of purified His-tag labeled protein, 3 * 30400, calculated mass of the His-tag labeled protein, native mass by gel filtration | Geobacter metallireducens |
Synonyms | Comment | Organism |
---|---|---|
dienoyl-CoA hydratase | - |
Syntrophus aciditrophicus |
dienoyl-CoA hydratase | - |
Desulfococcus multivorans |
dienoyl-CoA hydratase | - |
Geobacter metallireducens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
175 | - |
cyclohexa-1,5-diene-1-carbonyl-CoA | pH 7.5, 30°C | Syntrophus aciditrophicus | |
280 | - |
cyclohexa-1,5-diene-1-carbonyl-CoA | pH 7.5, 30°C | Geobacter metallireducens |