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Literature summary for 4.2.1.100 extracted from

  • Lampe, D.; Eisenreich, W.; Bacher, A.; Fuchs, G.
    Cyclohexa-1,5-diene-1-carboxyl-CoA hydratase, an enzyme involved in anaerobic metabolism of benzoyl-CoA in the denitrifying bacterium Thauera aromatica (1998), Eur. J. Biochem., 255, 618-627.
    View publication on PubMed
Search for more literature for 4.2.1.100

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
60
-
 cyclohexa-1,5-diene-1-carbonyl-CoA
-
 Thauera aromatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 alpha2, 2 * 28000, SDS-PAGE Thauera aromatica
55000
-
 gel filtration Thauera aromatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O Thauera aromatica
-
 6-hydroxycyclohex-1-enecarbonyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Thauera aromatica
-
 denitrifying bacterium, inducible enzyme
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose, hydroxyapatite, Mono-Q, Blue-Sepharose Thauera aromatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
-
 Thauera aromatica 6-hydroxycyclohex-1-ene-1-carbonyl-CoA
-
 r
cyclohexa-1,5-diene-1-carbonyl-CoA + H2O
-
 Thauera aromatica 6-hydroxycyclohex-1-enecarbonyl-CoA
-
 ?

Subunits

Subunits Comment Organism
dimer alpha2, 2 * 28000, SDS-PAGE Thauera aromatica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
51
-
 cyclohexa-1,5-diene-1-carbonyl-CoA
-
 Thauera aromatica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.6
-
 Thauera aromatica