Any feedback?
Literature summary for 4.2.1.10 extracted from
- A novel 3-dehydroquinate dehydratase catalyzing extracellular formation of 3-dehydroshikimate by oxidative fermentation of Gluconobacter oxydans IFO 3244 (2008), Biosci. Biotechnol. Biochem., 72, 1475-1482.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
3-dehydroquinate | apparent Km, pDQD | Gluconobacter oxydans |  |
| 0.05 | - |
3-dehydroquinate | apparent Km, sDQD | Gluconobacter oxydans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | cDQD | Gluconobacter oxydans | 5737 | - |
| membrane | pDQD | Gluconobacter oxydans | 16020 | - |
| periplasm | pDQD | Gluconobacter oxydans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | - |
pDQD, determined by SDS-PAGE, suggesting a multisubunit structure composed of 12 subunits | Gluconobacter oxydans |
| 16270 | - |
putative deduced molecular weight | Gluconobacter oxydans |
| 47000 | - |
sDQD, determined by SDS-PAGE, suggesting a multisubunit structure composed of eight subunits | Gluconobacter oxydans |
| 47270 | - |
putative deduced molecular weight | Gluconobacter oxydans |
| 189000 | - |
sDQD, determined by gel filtration | Gluconobacter oxydans |
| 191000 | - |
pDQD, determined by gel filtration | Gluconobacter oxydans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-dehydroquinate | Gluconobacter oxydans | - |
3-dehydroshikimate + H2O | - |
? | |
| 3-dehydroquinate | Gluconobacter oxydans IFO 3244 | - |
3-dehydroshikimate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | - |
- |
- |
| Gluconobacter oxydans IFO 3244 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| periplasmic and cytosolic 3-dehydroquinate dehydratases are purified using a DEAE-cellulose, a DEAE-Sephadex A-50, a Hydroxyapatite and a Sephadex G-200 column | Gluconobacter oxydans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0002 | - |
sDQD, cell-free extract | Gluconobacter oxydans |
| 0.0036 | - |
sDQD, purification step DEAE-cellulose | Gluconobacter oxydans |
| 0.02 | - |
pDQD, membrane fraction | Gluconobacter oxydans |
| 0.07 | - |
pDQD, solubilizez fraction | Gluconobacter oxydans |
| 0.09 | - |
pDQD, purification step DEAE-cellulose | Gluconobacter oxydans |
| 0.2 | - |
sDQD, purification step DEAE-Sephadex A-50 | Gluconobacter oxydans |
| 0.28 | - |
pDQD, purification step DEAE-Sephadex A-50 | Gluconobacter oxydans |
| 0.45 | - |
sDQD, purification step Sephadex G-200 | Gluconobacter oxydans |
| 0.83 | - |
sDQD, heat treatment | Gluconobacter oxydans |
| 0.97 | - |
pDQD, purification step Hydroxyapatite | Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-dehydroquinate | - |
Gluconobacter oxydans | 3-dehydroshikimate + H2O | - |
? | |
| 3-dehydroquinate | - |
Gluconobacter oxydans IFO 3244 | 3-dehydroshikimate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | - |
Gluconobacter oxydans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-dehydroquinate dehydratase | - |
Gluconobacter oxydans |
| DQD | - |
Gluconobacter oxydans |
| pDQD | periplasmic 3-dehydroquinate dehydratase | Gluconobacter oxydans |
| sDQD | soluble, cytoplasmic 3-dehydroquinate dehydratase | Gluconobacter oxydans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
activity assay | Gluconobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | activity assay | Gluconobacter oxydans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
